Rat Recombinant Mn SOD Protein
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Catalog numberSPR-130B
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PricePlease ask
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Size100 µg
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Stock availabilityIn Stock
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Scientific contextSuperoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(8). SOD3 can also be distinguished by its heparin-binding capacity (1).
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Protein targetMn SOD
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Protein reactivityRat
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Certificate of analysisThis product has been certified >90% pure using SDS-PAGE analysis.
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Protein descriptionRat Recombinant Mn SOD Protein
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Other nameManganese SOD Protein, IPO B Protein, Mn SOD Protein, SOD2 Protein
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Primary research areaCancer, Oxidative Stress, Cell Signaling, Trafficking, Chaperones
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CategoryProtein
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Brand namenone
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OriginRecombinant
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NCBI numberBC070913
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Gene number24787
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Protein numberP07895
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Verified applicationsWB, SDS-PAGE
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Relevant bio activityMn SOD Protein
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Protein expression modelE. coli
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Protein charastericsSee included datasheet.
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Peptide sequenceSee included datasheet.
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Protein purificationAffinity Purified
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Purity pourcentage>90% High purity
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Recommended buffer for storage50mM Tris/HCl pH7.7, 0.3M NaCl
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Protein concentrationLot/batch specific. See included datasheet.
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Protein specificity~25 kDa
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Protein tagHis tag
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Supplementary useful informationNo leader sequence
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Protein cell localizationMitochondrion Matrix
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Bibliography1. Adachi T., et al. (1992) Clin. Chim. Acta. 212: 89-102. 2. Barrister J.V., et al. (1987) Crit. Rev. Biochem. 22: 111-180. 3. Furukawa Y., and O’Halloran T. (2006) Antioxidants &Redo Signaling. 8 (No 5): 6. 4. Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol. 284: L917-L925. 5. Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe N., et al. (1990) Clinica Chimica Acta. 192: 171-180. 7. Ojika T., et al. (1991) Acta Histochem Cytochem. 24(50): 489-495. 8. Wispe J.R., et al. (1989) BBA. 994: 30-36.
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Release date8-Jun-2009
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PubMed numberNot added. Please refer to PubMed
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Tested applicationsto be tested
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Tested species reactivityto be tested
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Representative figure legendSDS-PAGE of 25kDa rat Mn SOD (SPR-130). SDS-Page of rat SOD (Mn) Protein (SPR-130)
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WarningsNon-hazardous materials
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Protein originCanada
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Total weight kg1.4
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Net weight g0.1
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolSOD3
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Short nameRecombinant Mn SOD Protein
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. StressMark proteins advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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HostRat
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SpeciesRat, Rats
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Alternative nameRat Rec. Mn antioxidant enzyme Protein
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namesuperoxide dismutase 3
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Synonyms gene name
- superoxide dismutase 3, extracellular
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Synonyms
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Locus
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Discovery year1988-05-08
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Entrez gene record
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Classification
- Superoxide dismutases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data