Rat Recombinant Mn SOD Protein

  • Catalog number
    SPR-130B
  • Price
    Please ask
  • Size
    100 µg
  • Stock availability
    In Stock
  • Scientific context
    Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(8). SOD3 can also be distinguished by its heparin-binding capacity (1).
  • Protein target
    Mn SOD
  • Protein reactivity
    Rat
  • Certificate of analysis
    This product has been certified >90% pure using SDS-PAGE analysis.
  • Protein description
    Rat Recombinant Mn SOD Protein
  • Other name
    Manganese SOD Protein, IPO B Protein, Mn SOD Protein, SOD2 Protein
  • Primary research area
    Cancer, Oxidative Stress, Cell Signaling, Trafficking, Chaperones
  • Category
    Protein
  • Brand name
    none
  • Origin
    Recombinant
  • NCBI number
    BC070913
  • Gene number
    24787
  • Protein number
    P07895
  • Verified applications
    WB, SDS-PAGE
  • Relevant bio activity
    Mn SOD Protein
  • Protein expression model
    E. coli
  • Protein charasterics
    See included datasheet.
  • Peptide sequence
    See included datasheet.
  • Protein purification
    Affinity Purified
  • Purity pourcentage
    >90% High purity
  • Recommended buffer for storage
    50mM Tris/HCl pH7.7, 0.3M NaCl
  • Protein concentration
    Lot/batch specific. See included datasheet.
  • Protein specificity
    ~25 kDa
  • Protein tag
    His tag
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Supplementary useful information
    No leader sequence
  • Protein cell localization
    Mitochondrion Matrix
  • Bibliography
    1. Adachi T., et al. (1992) Clin. Chim. Acta. 212: 89-102. 2. Barrister J.V., et al. (1987) Crit. Rev. Biochem. 22: 111-180. 3. Furukawa Y., and O’Halloran T. (2006) Antioxidants &Redo Signaling. 8 (No 5): 6. 4. Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol. 284: L917-L925. 5. Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe N., et al. (1990) Clinica Chimica Acta. 192: 171-180. 7. Ojika T., et al. (1991) Acta Histochem Cytochem. 24(50): 489-495. 8. Wispe J.R., et al. (1989) BBA. 994: 30-36.
  • Release date
    8-Jun-2009
  • PubMed number
    Not added. Please refer to PubMed
  • Tested applications
    to be tested
  • Tested species reactivity
    to be tested
  • Representative figure legend
    SDS-PAGE of 25kDa rat Mn SOD (SPR-130). SDS-Page of rat SOD (Mn) Protein (SPR-130)
  • Warnings
    Non-hazardous materials
  • Protein origin
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • About
    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Rattus norvegicus
  • Source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    SOD   Protein  
  • Gene symbol
    SOD3
  • Short name
    Recombinant Mn SOD Protein
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. StressMark proteins advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Host
    Rat
  • Species
    Rat, Rats
  • Alternative name
    Rat Rec. Mn antioxidant enzyme Protein
  • Alternative technique
    rec
Gene info
  • Identity
  • Gene
  • Long gene name
    superoxide dismutase 3
  • Synonyms gene name
    • superoxide dismutase 3, extracellular
  • Synonyms
  • Locus
  • Discovery year
    1988-05-08
  • Entrez gene record
  • Classification
    • Superoxide dismutases
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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