Human Recombinant HSP60 Protein

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• Catalog number
  SPR-104B
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• Size
  100 µg

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• Stock availability
  In Stock
• Scientific context
  In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
• Protein target
  HSP60
• Protein reactivity
  Human
• Certificate of analysis
  This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 3.6µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
• Protein description
  Active Human Recombinant HSP60 Protein
• Other name
  60kDa chaperonin Protein, cb863 Protein, CPN60 Protein, GROEL Protein, GroEL Homolog Protein, HLD4 Protein, HSP65 Protein, HSPD1 Protein, HuCHA60 Protein, SPG13 Protein
• Primary research area
  Cancer, Heat Shock
• Category
  Protein
• Brand name
  none
• Origin
  Recombinant
• NCBI number
  BC003030
• Gene number
  3329
• Protein number
  P10809
• Verified applications
  WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA
• Relevant bio activity
  ATPase active
• Protein expression model
  E. coli
• Protein charasterics
  See included datasheet.
• Peptide sequence
  See included datasheet.
• Protein purification
  Affinity Purified
• Purity pourcentage
  >90% High purity
• Recommended buffer for storage
  20mM Phosphate Buffer, 150mM NaCl, 10% glycerol
• Protein concentration
  Lot/batch specific. See included datasheet.
• Protein specificity
  ~60 kDa
• Protein tag
  His tag
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Supplementary useful information
  Please see included datasheet or contact us
• Protein cell localization
  Mitochondrion Matrix
• Bibliography
  1. Hartl F.U. (1996) Nature. 381: 571-579. 2. Bukau B. and Horwich A.L. (1998) Cell. 92: 351-366. 3.Hartl F.U. and Hayer-Hartl M. (2002) Science. 295: 1852-1858. 4. Jindal S., et al. (1989) Molecular and Cellular Biol. 9: 2279-2283. 5. La Verda D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71. 6. Itoh H., et al. (2002) Eur. J. Biochem. 269: 5931-5938. 7.Gupta S. and Knowlton A.A. J. Cell Mol Med. 9: 51-58. 8. Deocaris C.C. et al. (2006) Cell Stress Chaperones. 11: 116-128. 9. Lai H.C., et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297.
• Release date
  1-Sep-2007
• PubMed number
  23775284, 24066722, 21506099
• Tested applications
  Functional Assay, Western Blot Control, ELISA
• Tested species reactivity
  Human
• Representative figure
• Representative figure legend
  SDS-PAGE of 60kDa Hsp60 protein (SPR-104). SDS-Page of human HSP60 Protein (SPR-104)
• Warnings
  Non-hazardous materials
• Protein origin
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Other related products
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

#

• Gene target
  HSP60   Protein  
• Gene symbol
  HSPD1
• Short name
  Recombinant HSP60 Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. StressMark proteins advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. HSP60 Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:5261
• Gene
  HSPD1
• Long gene name
  heat shock protein family D (Hsp60) member 1
• Synonyms gene
  • SPG13
• Synonyms gene name
  • heat shock 60kD protein 1 (chaperonin)
  • spastic paraplegia 13 (autosomal dominant)
  • heat shock 60kDa protein 1 (chaperonin)
• Synonyms
  • GroEL
  • HSP60
• GenBank acession
  • M34664
• Locus
  2q33.1
• Discovery year
  1991-07-19
• Entrez gene record
  3329
• Pubmed identfication
  • 1980192
  • 11898127
• RefSeq identity
  • NM_002156
• Classification
  • Chaperonins
• VEGA ID
  OTTHUMG00000154463

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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