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Stock availability
In Stock
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Kit s description
Quantitative colorimetric measurement of PGE2
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Protein target
Prostaglandin E2
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Scientific context
Eicosanoid signal transduction pathways are highly conserved and are involved in a number of physiological processes. Prostaglandins are synthesized from arachidonic acid by cyclooxygenase (COX)-1 or -2, which convert the acid into PGH2. This is further processed by cytosolic or microsomal prostaglandin synthases to become PGE2 or one of several other prostanoids (1-3). Prostacyclin is the major cyclooxygenase product in blood vessel walls and it is present in inflammatory fluids in similar concentrations to PGE2. Prostacyclin is a potent vasodilator and is more potent than PGE2 in producing hyperalgesia (4). PGE2 is produced by a wide variety of tissues (5-12) and in several pathological conditions, including inflammation, arthritis, fever, tissue injury, endometriosis, and a variety of cancers (5,6). Other biological actions of PGE2 include vasodilation, modulation of sleep/wake cycles, and facilitation of human immunodeficiency virus replication. It elevates cAMP levels, stimulates bone resorption, and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics (7-12).
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Primary research area
Cell Signaling, Cancer, Cardiovascular System
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Category
Assay EIA Kits
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Other name
(5Z,11α,13E,15S)-7-[3-hydroxy-2-(3-hydroxyoct-1-enyl)- 5-oxo-cyclopentyl] hept-5-enoic acid EIA Kit
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Brand name
StressXpress®
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Detection system
Colorimetric Assay
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Assay format
Sandwich EIA (Enzyme Immunoassay)
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Main intended usage
EIA kit used to quantitatively measure PGE2 present in samples.
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Brief protocol
The Prostaglandin E2 (PGE2) EIA kit is designed to quantitatively measure PGE2 present in serum, plasma, urine, saliva, cells, tissue, and tissue culture media samples. This EIA kit allows for the widest variations in sample size, sensitivity and assay timing of any PGE2 kit. The protocol variations are outlined below: The Regular Format, which uses 50 µL of sample or standard, is shown on pages 10-11, gives results in 2.5 hours. The Low Sample Volume Format, which uses 25 µL of sample or standard, is outlined on page 12-13, also gives results in 2.5 hours, but uses lower sample volumes. The High Sensitivity Format, which uses 100 µL of sample or standard, is shown on page 14-15, also gives results in 2.5 hours, but is the highest sensitivity kit of any type available. The Overnight Format, which uses 50 µL of sample or standard, is shown on page 16-17, and uses a primary overnight incubation at 4°C to fit your work flow. This format has similar sensitivity to the Regular Format outlined above.
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Kit contents
Coated White 96 Well Plates, Prostaglandin E2 Standard, StressXpress® Prostaglandin E2 Antibody, StressXpress® Prostaglandin E2 Conjugate, Assay Buffer, Wash Buffer Concentrate, TMB Solution, Stop Solution, Plate Sealer
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Assay precision
Intra Assay Precision: Three human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Prostaglandin E2 concentrations were: Sample 1- 11.7 pg/mL, 12.3% CV Sample 2- 98.6 pg/mL, 6.3% CV Sample 3- 131.2 pg/mL, 4.9% CV Inter Assay Precision: Three human samples were diluted with Assay Buffer and run in duplicates in seventeen assays run over multiple days by four operators. The mean and precision of the calculated Prostaglandin E2 concentrations were: Sample 1- 12.3 pg/mL, 8.8% CV Sample 2- 100.5 pg/mL, 8.1% CV Sample 3- 134.7 pg/mL, 9.8% CV
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Assay cross reactivity
Species Independent
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Samples to be used with this kit
Saliva, Urine, Serum, EDTA Plasma, Heparin Plasma, Tissue Culture Media
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Maximum samples to be used with this kit
40 samples in duplicate
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Assay duration
See product datasheet or feel free to contact us
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Kit s sensitivity
3,07 pg/ml
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Assay detection limit
HS: 1,953 - 500 pg/ml, LV: 15,625 - 1000 pg/ml, RG: 3,906 - 500 pg/ml, ON: 3,906 - 500 pg/ml,
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice
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Bibliography
1. Moncada, S., Ferriera, SH. & Vane, JR. (1979). Handbook of Experimental Pharmacology,50/II. Pp. 588-616. Vane, J.R. & Ferreira, S.H. Berlin,New York: Springer. 2. Vane, JR. (1971). Nature,231, 232-235.3. 3. Willis, AL. (1969). In Prostaglandins, Peptides and Amines.Pp. 31-38. Ed. Mantegazza, P. & Horton, E.W.London: Academic Press. 4. Higgs, GA., Cardinal, DC., Moncada, S. & Vane, JR. (1979). Microvascular Res., 18,245-254. 5. Kargman, S. et al. (1996) Biochem Pharmacol. 52(7):1113-25 6. Thun MJ, Namboodiri MM, Heath CW Jr. New Engl. J. Med. (1991) 325: 1593–6. 7. Richardson PD and Withrington PG. Brit. J. Pharmacol. (1976) 57: 581-588. 8. O. Hayaishi. J. Biol. Chem. (1988) 263: 14593-14596. 9. S. Kuno, et al. Proc. Natl. Acad. Sci., USA, (1986) 83: 3487-3490. 10. D.L. Bareis, et al. Proc. Natl. Acad. Sci., USA, (1983) 80: 2514-2518. 11. L.G. Raisz, et al. Nature. (1977) 267: 532-534. 12. C.R. Long, Kinoshita Y, Knox FG. Prostaglandins. (1990) 40: 591-601.
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Release date
17-Sep-2014
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PubMed number
Refer to PubMed
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Tested applications
No data available / currently testing
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Tested reactivity
No data available / currently testing
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Resources available upon request
Kit Booklet, MSDS, Saliva Sample Handling Instructions, Eicosanoid Sample Extraction Protocol
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Product image link
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Product image legend
Regular Format Typical Standard Curve for the Prostaglandin E2 EIA Kit (Enzyme Immunoassay) StressXpress® - SKT-207. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: HS: 1.953 - 500 pg/ml. LV: 15.625 - 1000 pg/ml. RG: 3.906 - 500 pg/ml. ON: 3.906 - 500 pg/ml. . | Multi-Format Standard Curve for the Prostaglandin E2 EIA Kit (Enzyme Immunoassay) StressXpress® - SKT-207. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: HS: 1.953 - 500 pg/ml. LV: 15.625 - 1000 pg/ml. RG: 3.906 - 500 pg/ml. ON: 3.906 - 500 pg/ml. . | Linearity was determined in human plasma and urine samples by taking two diluted samples with known PGE2 concentrations. A plasma sample with a high PGE2 concentration of 216.4 pg/mL was mixed with one with a lower value of 42.5 pg/mL. A urine sample with a high PGE2 concentration of 32.6 pg/mL was mixed with one with a lower value of 8.6 pg/mL. They were mixed in the ratios. The measured concentrations were compared to the expected values based on the ratios used. Graph of the Regular Format Typical Standard Curve for the Prostaglandin E2 EIA Kit StressXpress - SKT-207 | Graph of the Multi-Format Standard Curve for the Prostaglandin E2 EIA Kit StressXpress - SKT-207 | Graph of the Plasma and Urine Linearity Recovery for the Prostaglandin E2 EIA Kit StressXpress - SKT-207
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Warnings
Non-hazardous
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Country of production
Canada
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Total weight kg
2
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Net weight g
300
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
