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Stock availability
In Stock
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Kit s description
Colorimetric detection of Nitrotyrosine
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Protein target
Nitrotyrosine
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Scientific context
Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present. Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.
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Primary research area
Cancer, Oxidative Stress, Cell Signaling, Post-translational Modifications, Nitration, Neuroscience, Neurodegeneration, Alzheimer's Disease, Parkinson's Disease
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Category
Assay ELISA Kits
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Other name
Nitro tyrosine ELISA Kit, 3-Nitrotyrosine ELISA Kit, 3-Nitro-L-tyrosine ELISA Kit, 3 Nitrotyrosine ELISA Kit, 3-NT ELISA Kit, 3NT ELISA Kit
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Brand name
MOLECULAR SIGNATURE®
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Detection system
Colorimetric Assay
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Assay format
Competitive ELISA (Enzyme-linked Immunosorbent Assay)
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Main intended usage
ELISA kit used to quantitate Nitrotyrosine in samples.
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Brief protocol
1. Prepare standard and samples in the Sample and Standard Diluent. 2. Add 50 µL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 µL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour. 5. Wash plate 4 times with 1X Wash Buffer. 6. Add 100 µL of TMB Substrate to each well. 7. Cover plate and develop the plate in the dark at room temperature for 30 minutes. 8. Add 100 µL of Stop Solution to each well. 9. Measure absorbance on a plate reader at 450 nm. 10. Plot the standard curve and calculate sample concentrations.
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Kit contents
Nitrosylated BSA Coated Plate, Nitrotyrosine Standard , Nitrotyrosine HRP Conjugated Monoclonal Antibody, , Sample and Standard Diluent (Red), Nitrotyrosine Antibody Diluent (Blue), Wash Buffer Concentrate (10X), TMB Substrate, Stop Solution, Plate Cover
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Assay precision
Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate. The intra-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be
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Assay cross reactivity
Species Independent
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Samples to be used with this kit
Plasma, Serum, Cell Lysates, Urine
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Maximum samples to be used with this kit
38 samples in duplicate
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Assay duration
1 hour
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Kit s sensitivity
50 nM
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Assay detection limit
62,5 - 8000 nM
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Storage recommendations
4°C
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Shipping recommendations
Blue Ice
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Bibliography
Refer to PubMed
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Release date
28-Sep-2015
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PubMed number
Refer to PubMed
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Tested applications
No data available / currently testing
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Tested reactivity
No data available / currently testing
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Resources available upon request
Kit Booklet, MSDS, Calculations Worksheet
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Product image link
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Product image legend
Typical Standard Curve for the Nitrotyrosine ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® - SKT-126. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 62.5 - 8000 nM. | Diagram of the Competitive ELISA | Diagram of the Preparation of Nitrotyrosine Standards | Diagram of the Triplicate Sample Plate Format Graph of the Typical Standard Curve for the Nitrotyrosine ELISA Kit StressXpress - SKT-126 | Diagram of the Competitive ELISA for the Nitrotyrosine ELISA Kit StressXpress - SKT-126 | Diagram of the Preparation of Nitrotyrosine Standards for the Nitrotyrosine ELISA Kit StressXpress - SKT-126 | Diagram of the Triplicate Sample Plate Format for the Nitrotyrosine ELISA Kit StressXpress - SKT-126
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Warnings
Non-hazardous
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Country of production
Canada
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Total weight kg
2
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Net weight g
2500
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Short name
Nitrotyrosine ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Alternative name
Nitrotyrosine Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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