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Stock availability
In Stock
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Kit s description
Colorimetric measurement of creatinine
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Protein target
Creatinine Serum
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Scientific context
Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP (1). Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist (2). Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo , this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine (3). Under normal conditions, its formation occurs at a rate that is relatively constant and as intra- individual variation is
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Primary research area
Cell Signaling, Cardiovascular System
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Category
Assay Detection Kits
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Other name
N-Carbamimidoyl-N-methylglycine Detection Kit, Methylguanidoacetic acid Detection Kit
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Brand name
StressXpress®
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Detection system
Colorimetric Assay
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Assay format
Direct Quantitative Assay
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Main intended usage
Colorimetric assay used to measure creatinine in samples.
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Brief protocol
The Creatinine Serum Detection Kit is designed to quantitatively measure creatinine present in serum samples. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or samples are pipetted into a clear microtiter plate. An assay diluent is added to all standards, controls and samples. The color generating reaction is initiated with the StressXpress® Creatinine Reagent, which is pipetted into each well. The assay utilizes a kinetic absorbance method to overcome interference by colored compounds in serum. The absorbance of the colored product is read after 1 minute in a microtiter plate reader capable of measuring 490nm wavelength. At 30 minutes the optical density is read again. The concentration of creatinine is calculated using the delta of the optical density readings at 30 and 1 minute compared to the curve generated from the standards, or by using the Excel worksheet available for free download at our web site. The Jaffe reaction used in this kit has been modified to read creatinine levels in serum,8.
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Kit contents
Clear 96 well Half Area Plates, Creatinine Standard, Assay Diluent, StressXpress® Creatinine Reagent
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Assay precision
Intra Assay Precision: Three human serum samples were run in replicates of 20 in an assay. The mean and precision of the calculated creatinine concentrations were: Sample 1- 0.99 mg/dL, 7.9% CV Sample 2- 1.50 mg/dL, 6.3% CV Sample 3- 3.82 mg/dL, 4.5% CV Inter Assay Precision: Three human serum samples were run in duplicates in 19 assays run over two years by four operators. The mean and precision of the calculated creatinine concentrations were: Sample 1- 0.91 mg/dL, 9.6% CV Sample 2- 1.26 mg/dL, 7.3% CV Sample 3- 3.51 mg/dL, 8.0% CV
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Assay cross reactivity
Human, Mouse, Rabbit, Rat, Sheep
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Samples to be used with this kit
Serum, Plasma
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Maximum samples to be used with this kit
91 samples in duplicate
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Assay duration
See product datasheet or feel free to contact us
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Kit s sensitivity
0,081 mg/dl
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Assay detection limit
0,5 - 4 mg/dl
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Storage recommendations
4°C
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Shipping recommendations
Blue Ice
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Bibliography
1. Wallimann, T. et al., Biochem. J., 2000, 281, 21-40. 2. Wyss, M. and Kaddurah-Daouk, R., Physiol. Rev., 2000, 80, 1107-1213. 3. Raja Iyengar, M. et al., J. Biol. Chem, 1985, 260, 7562-7567. 4. Manjunath, G. et al., Postgrad. Med. 2001, 110, 55-62. 5. Gross, J.L. et al., Diabetes Care, 2005, 28, 164-176. 6. Anavekar, N.S. et al., New Engl. J. Med., 2004, 351, 1285-1295.
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Release date
17-Sep-2014
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PubMed number
Refer to PubMed
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Tested applications
No data available / currently testing
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Tested reactivity
No data available / currently testing
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Resources available upon request
Kit Booklet, MSDS
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Product image link
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Product image legend
Typical Standard Curve for Creatinine Serum Detection Kit StressXpress® - SKT-217. Assay Type: Direct Enzyme. Detection Method: Colorimetric Assay. Assay Range: 0.5 - 4 mg/dl. | Linearity was determined by taking two human serum samples, one with a low diluted creatinine level of 0.75 mg/dL and one with a higher level of 3.78 mg/dL and mixing them in given ratios. The measured concentrations were compared to the expected values. | Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine. Graph of the Typical Standard Curve for the Creatinine Serum Detection Kit StressXpress - SKT-217 | Graph of the Linearity Recovery for the Creatinine Serum Detection Kit StressXpress - SKT-217 | Chemical Equation of the Creatinine Formation for the Creatinine Serum Detection Kit StressXpress - SKT-217
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Warnings
Non-hazardous
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Country of production
Canada
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Total weight kg
2
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Net weight g
300
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Description
The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
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About
Serum should be stored at +5°C
