Rabbit Anti-Rat PDI Polyclonal: PE/ATTO 594

  • Catalog number
    SPC-114D-P594
  • Price
    Please ask
  • Size
    100 µl
  • Immunogen
    Rat PDI synthetic peptide conjugated to KLH
  • Antibody s target
    Rat PDI
  • Antibody s full description
    Rabbit Anti-Rat PDI Polyclonal
  • Primary research fields
    Cell Signaling, Trafficking, Chaperones, Organelle Markers
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    PDA2 Antibody, PDI Antibody, PDIA2 Antibody, PDIP Antibody, pancreatic protein disulfide isomerase Antibody
  • Verified applications
    WB, IHC, ICC/IF, IP
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human, Mouse, Rat, Bovine, Dog, Hamster, Monkey, Guinea Pig (Cavia porcellus), Sheep, Pig, African clawed frog (Xenopus laevis), Mollusk, Mussel (Perna viridis)
  • Antibody s recommended dilutions for use
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~58kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-114 was sufficient for detection of PDI in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
  • Antibody in cell
    Endoplasmic Reticulum, Endoplasmic Reticulum Lumen
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5). PDI is an abundant 55kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).
  • Bibliography
    1. NA K.S. et al. (2007) Mol Cells. 24(2): 261-7. 2. Mayer M., Kies U., Kammermeier R., and Buchner J. (2000) J Biol Chem. 275(38): 29421-5. 3. Delom F., Mallet B., Carayon P., and Lejeune P.J. (2001) J Biol Chem 276(24): 21337-42. 4. Schultz-Norton J.R., McDonald W.H., Yates J.R. and Nardulli A.M. (2006) Mol Endocrinol 20(9): 1982-95. 5. Turano C., Coppari S. Altieri F. and Ferraro (2002) J Cell Physiol 193: 154-163. 6. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813- 40819. 7. Yoshimori T., et al. (1990) J Biol Chem. 265(26): 15984-90.
  • Released date
    1/Jun/2008
  • NCBI number
    NP_001099245.2
  • Gene number
    287164
  • Protein number
    P04785
  • PubMed number
    25678563|25678563|21684241
  • Tested applications
    Western Blot|Western Blot|Western Blot
  • Tested reactivity
    Human|Mouse|Perna viridis (Asian green mussel)
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PDI Polyclonal Antibody (SPC-114). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (SPC-114) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-PDI Antibody. (C) Composite. | Western blot analysis of Rat tissue mix showing detection of PDI protein using Rabbit Anti-PDI Polyclonal Antibody (SPC-114). Load: 15 µg protein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (SPC-114) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PDI Polyclonal Antibody (SPC-114). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (SPC-114) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-PDI Antibody. (C) Composite. Rabbit Anti-PDI Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-114) | Rabbit Anti-PDI Antibody used in Western blot (WB) on Rat tissue mix (SPC-114) | Rabbit Anti-PDI Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-114)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Oryctolagus cuniculus, Rattus norvegicus
  • Gene target
    PDI   PE/ATTO   594  
  • Gene symbol
    PDIA2, PADI1, ERP29, P4HB
  • Short name
    Rabbit Anti- PDI Polyclonal: PE/ATTO 594
  • Technique
    Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    PE/ATTO 594
  • Species
    Rat, Rats
  • Alternative name
    production species: rabbit Antibody toRat PDI polyclonal: peroxidase/ATTO 594
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
Gene info
  • Identity
  • Gene
  • Long gene name
    protein disulfide isomerase family A member 2
  • Synonyms gene
  • Synonyms gene name
    • protein disulfide isomerase, pancreatic
    • protein disulfide isomerase-associated 2
    • protein disulfide isomerase family A, member 2
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-12-21
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Protein disulfide isomerases
  • VEGA ID
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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