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Immunogen
A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
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Antibody s target
Rat ERK1
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Antibody s full description
Rabbit Anti-Rat Erk1/2 Polyclonal
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Primary research fields
Cell Signaling, Post-translational modifications, Phosphorylation, Neuroscience, Neurodegeneration, Alzheimer's Disease
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ERK1 Antibody, ERK2 Antibody, ERT1 Antibody, ERT2 Antibody, MAP kinase1 Antibody, MAP kinase2 Antibody, MAPK1 Antibody, MAPK2 Antibody, MAPK3 Antibody, p38 Antibody, p40 Antibody, p41 Antibody, p41mapk Antibody, p42 MAPK Antibody, p44 ERK1 Antibody, p44 MAPK Antibody, PRKM1 PRKM2 Antibody, PRKM3 Antibody
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Verified applications
WB, IHC, ICC/IF, FCM
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat, Bovine, Sheep, Chicken, Fruit Fly (Drosophila melanogaster), African clawed frog (Xenopus laevis)
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ICC/IF (1:100), FCM (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~44kda (ERK1) and ~42kDa (ERK2).
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-120 was sufficient for detection of ERK1/2 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
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Antibody in cell
Cytoplasm, Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5); however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).
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Bibliography
1. Boulton TG. et al. (1991) Biochemistry. 30(1):278-86. 2. Yoon S., and Seger R. (2006) Growth Factors 24:21-44. 3. Wolf G. (2005) Antioxid Redox Signal 7:1337-1345. 4. Chuerland D., Marmor G., Shainskaya A. and Seger R. (2008) J Biol Chem. Epub: more 5. Zhuang S., and Schnellmann R.G. (2006) J Pharmacol Exp Ther 319:991-997.
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Released date
1/May/2008
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NCBI number
NP_059043.1
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Gene number
50689
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Protein number
P21708
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PubMed number
26828753|25968337|25403445|20096933|20106878
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Tested applications
|Western Blot|Western Blot|Western Blot|Immunohistochemistry
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Tested reactivity
|Mouse|Human|Chicken|Mouse
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal Antibody (SPC-120). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody (SPC-120) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite. | Western blot analysis of Human Cell line lysates showing detection of ERK1 protein using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120) at 1:25000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus. | Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal Antibody (SPC-120). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody (SPC-120) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite. Rabbit Anti-Erk1/2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-120) | Rabbit Anti-ERK1 Antibody used in Western blot (WB) on Human Cell line lysates (SPC-120) | Rabbit Anti-ERK1 Antibody used in Immunohistochemistry (IHC) on Mouse Inflamed colon (SPC-120) | Rabbit Anti-ERK1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SPC-120) | Rabbit Anti-ERK1 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-120) | Rabbit Anti-Erk1/2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-120)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Oryctolagus cuniculus, Rattus norvegicus
