Rabbit Anti-Pseudomonas aeruginosa Metallothionein Polyclonal: APC

  • Catalog number
    SPC-717D-APC
  • Price
    Please ask
  • Size
    100 µg
  • Immunogen
    Synthetic peptide from the mid-protein of Pseudomonas aeruginosa Metallothionein
  • Antibody s target
    Pseudomonas aeruginosa Metallothionein
  • Antibody s full description
    Rabbit Anti-Pseudomonas aeruginosa Metallothionein Polyclonal
  • Primary research fields
    Cell Signaling, Epigenetics and Nuclear Signalling, Transcription, Domain Families, Zinc finger
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    CES 1 antibody, CES1 antibody, Metallothionein 1A antibody, Metallothionein 1S antibody, Metallothionein 2 antibody, Metallothionein 2A antibody, Metallothionein IA antibody, Metallothionein II antibody, Metallothionein-1A antibody, Metallothionein-IA antibody, Metallothionein2 antibody, MGC32848 antibody, MT 1A antibody, MT 2 antibody, MT 2A antibody, MT IA antibody, MT II antibody, MT-1A antibody, MT-IA antibody, MT1 antibody, MT1A antibody, MT1A_HUMAN antibody, MT1S antibody, MT2 antibody, MT2A antibody, MTC antibody, Probable Metallothionein Antibody
  • Verified applications
    WB
  • Raised in
    Rabbit
  • Antibody s reactivity
    Bacteria (Pseudomonas aeruginosa)
  • Antibody s recommended dilutions for use
    WB (1:1000); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~36 kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-717 was sufficient for detection of Metallothionein in 15 µg of pseudomonas aeruginosa purified Metallothionein (pmtA-GST) protein by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Contact our support service.
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    See included datasheet or contact our support service.
  • Bibliography
    Refer to PubMed
  • Released date
    25/Oct/2016
  • NCBI number
    Refer to NCBI
  • Gene number
    Refer to GenBank
  • Protein number
    Q9I1X5
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure legend
    Western blot analysis of Pseudomonas aeruginosa Purified protein showing detection of ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST) Metallothionein protein using Rabbit Anti-Metallothionein Polyclonal Antibody (SPC-717). Lane 1: Molecular Weight Ladder (MW). Lane 2: Blank. Lane 3: Pseudomonas aeruginosa purified protein with GST tag.. Load: 25 µg. Block: 1% milk, 2% BSA. Primary Antibody: Rabbit Anti-Metallothionein Polyclonal Antibody (SPC-717) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Mouse Anti-Rabbit IgG: AP at 1:1000 for 60 min at RT. Color Development: Alkaline phosphate activity colorimetric assay. Predicted/Observed Size: ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST). Courtesy of: Michael Lynes and Amy Thees, University of Connecticut. Rabbit Anti-Metallothionein Antibody used in Western blot (WB) on Purified protein (SPC-717)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Pseudomonas is a Gram-negative bacteria. Pseudomonas genomic sequences are used in medical research. A problem in hostpital is often the Pseudomonas Aeruginosa containing multi drug resistancy properties that are transmitted through plasmids. The MDR property is transmitter by the R plasmid. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
  • Gene symbol
    APC
  • Short name
    Rabbit Anti-Pseudomonas aeruginosa Metallothionein Polyclonal: APC
  • Technique
    Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    APC
  • Species
    Pseudomonas, Pseudomonas
  • Alternative name
    production species: rabbit Antibody toPseudomonas aeruginosa Metallothionein polyclonal: adenomatous polyposis coli
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
  • Alternative to gene target
    adenomatous polyposis coli, BTPS2 and DP2 and DP2.5 and DP3 and GS and PPP1R46, APC and IDBG-37007 and ENSG00000134982 and 324, microtubule plus-end binding, nuclei, Apc and IDBG-134573 and ENSMUSG00000005871 and 11789, BT.25770 and IDBG-644937 and ENSBTAG00000018852 and 533233
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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