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Immunogen
Phosphotyrosine conjugated to KLH
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Antibody s target
Phosphotyrosine
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Antibody s full description
Rabbit Anti-Phosphotyrosine Polyclonal
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Primary research fields
Cell Signaling, Post-Translational Modifications, Phosphorylation
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Phospho-tyrosine Antibody
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Verified applications
WB, IHC, ICC/IF, IP, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Species Independent
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Antibody s recommended dilutions for use
WB (1:500), IHC (1:100), ICC/IF (1:50), ELISA (1:2000), IP (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS pH7.0, 0.01% sodium azide
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Antibody s concentration
0.25 mg/ml
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Antibody s specificity
Detects proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine.
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Storage recommendations
4°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-161 was sufficient for detection of tyrosine-phosphorylated species in mouse spleen lysates in western blot analysis.
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Antibody in cell
Contact our support service.
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).
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Bibliography
1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352. 6. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656. 7. Ostrovsky PC. (1995) Genes Dev. 9(16): 2034-2041.
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Released date
1/Jun/2007
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
Refer to Swiss-Prot
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161) at 1:50 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite. | Immunohistochemistry analysis using Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161). Tissue: breast cancer. Species: Human . Primary Antibody: Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161) at 1:100. Rabbit Anti-Phosphotyrosine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-161) | Rabbit Anti-Phosphotyrosine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-161) | Rabbit Anti-Phosphotyrosine Antibody used in Immunohistochemistry (IHC) on Human breast cancer (SPC-161)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.4
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
