Rabbit Anti-Phosphothreonine Polyclonal: FITC

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• Catalog number
  SPC-154F-FITC
• Price:

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• Size
  400 µl

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• Immunogen
  Phosphothreonine conjugated to KLH
• Antibody s target
  Phosphothreonine
• Antibody s full description
  Rabbit Anti-Phosphothreonine Polyclonal
• Primary research fields
  Cell Signaling, Post-Translational Modifications, Phosphorylation
• Antibody s category
  Polyclonal Antibodies
• Antibody s other name
  Phospho-threonine Antibody
• Verified applications
  WB, ICC/IF, IP, ELISA
• Raised in
  Rabbit
• Antibody s reactivity
  Species Independent
• Antibody s recommended dilutions for use
  WB (1:500), ICC/IF (1:60), ELISA (1:2000), IP (1:100); optimal dilutions for assays should be determined by the user.
• Antibody s purified from
  Protein A Purified
• Recommended buffer for storage
  PBS, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  0.25 mg/ml
• Antibody s specificity
  Detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Certificate of analysis
  2 µg/ml of SPC-154 was sufficient for detection of phosphorylation signal in western blot analysis using mouse spleen extract treated with Vanadium.
• Antibody in cell
  Contact our support service.
• Tissue specificity
  See included datasheet or contact our support service.
• Scientific context
  Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
• Bibliography
  1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Ostrovsky P.C. (1995) Genes Dev. 9(16): 2034-2041.
• Released date
  1/Jun/2007
• NCBI number
  Refer to NCBI
• Gene number
  Refer to GenBank
• Protein number
  Refer to Swiss-Prot
• PubMed number
  19452503
• Tested applications
  Western Blot
• Tested reactivity
  Human
• Antibody s datasheet
  Contact our support service to receive datasheet or other technical documentation.
• Representative figure link
  more | details | see here
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphothreonine Polyclonal Antibody (SPC-154). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody (SPC-154) at 1:60 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite. | Western blot analysis of Mouse brain cell lysates showing detection of Phosphothreonine protein using Rabbit Anti-Phosphothreonine Polyclonal Antibody (SPC-154). Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody (SPC-154) at 1:1000. Left: Treated with Vanadium, Right: Non-treated.. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphothreonine Polyclonal Antibody (SPC-154). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody (SPC-154) at 1:60 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite. Rabbit Anti-Phosphothreonine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-154) | Rabbit Anti-Phosphothreonine Antibody used in Western blot (WB) on Mouse brain cell lysates (SPC-154) | Rabbit Anti-Phosphothreonine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-154)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.4
• Stock availabilit
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

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• Gene target
  Phosphothreonine  
• Short name
  Rabbit Anti-Phosphothreonine Polyclonal: FITC
• Technique
  Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  Immunoglobulin
• Label
  FITC
• Alternative name
  production species: rabbit Antibody toPhosphothreonine polyclonal: fluorecein
• Alternative technique
  polyclonals, rabbit-anti, antibodies, fluorescine

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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