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Immunogen
Peptide corresponding to AA 20-43 of the mouse TNF-R1 sequence, identical to rat and human over those residues
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Antibody s target
Mouse TNFR1
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Antibody s full description
Rabbit Anti-Mouse TNF-R1 Polyclonal
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Primary research fields
Cancer, Apoptosis, Cell Signaling, Cardiovascular System, Atherosclerosis
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Tumor necrosis factor receptor 1 Antibody, TNFR-1 Antibody, TNFRSF1A Antibody, TNFAR Antibody, TNFR1 Antibody
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Verified applications
WB, IHC, ICC/IF, IP
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat, Bovine, Monkey, Dog, Rabbit
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~55kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
1 µg/ml of SPC-170 was sufficient for detection of TNFR1 in 20 µg of Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Membrane, Golgi Apparatus, Golgi Apparatus Membrane
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
The Tumor Necrosis Factor Receptor (TNFR) also known as Cluster of differentiation (CD120) is a protein that belongs to the (TNF)/ (TNFR) superfamily. TNF interacts with two distinct receptors TNFR1 and TNFR2. These receptors share no homology on their cytoplasmic sequences(1,3).TNFR1 also known as p55/p60 is a high affinity receptor for TNF-α. The TNFR1 has an extracellular domain with variable numbers of cysteine-rich repeats. The functional properties of TNFR1 are targets in new therapies for osteoporosis, chronic inflammatory and autoimmune diseases (1, 2). The TNF-α/TNFR1 receptor complex is responsible for the recruitment and the subsequent activation of the caspase (aspartate-specific cysteine proteases) that regulate apoptosis.
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Bibliography
1. Kontermann R.E., et al. (2008) J Immunother. 31(3):225-34. 2. Hehlgans T. and Pfeffer K. (2005) Immunology. 115(1):1-20. 3. Al-Lamki S., et al. (2005) The Faseb Journal. 19:1638-1645.
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Released date
9/May/2013
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NCBI number
P19438
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Gene number
9606
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Protein number
P19438
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Golgi apparatus membrane. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TNF-R1 Antibody. (C) Composite. | Immunohistochemistry analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: dermis. | Western blot analysis of Mouse Liver cell lysates showing detection of ~55 kDa TNF-R1 protein using Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55 kDa. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Punctate nuclear staining, dotty staining in cytoplasm. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Golgi apparatus membrane. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-TNF-R1 Antibody. (C) Composite. Rabbit Anti-TNF-R1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-170) | Rabbit Anti-TNF-R1 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-170) | Rabbit Anti-TNF-R1 Antibody used in Western blot (WB) on Liver cell lysates (SPC-170) | Rabbit Anti-TNF-R1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SPC-170) | Rabbit Anti-TNF-R1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-170)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Oryctolagus cuniculus
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Gene
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
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Gene target
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Gene symbol
TNFRSF1A, TNF, C17orf75, CDCA7L, TNFRSF1B, CNEP1R1, LTBR, CXCL11, PNPLA7, PCAT6
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Short name
Rabbit Anti-Mouse TNF-R1 Polyclonal: ATTO 655
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Technique
Polyclonal, Rabbit, anti-, Mouse, anti, antibody to, antibodies, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
ATTO 655
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Species
Mouse, Mouses
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Alternative name
production species: rabbit Antibody toMouse tumor necrosis factor-R1 polyclonal: ATTO 655
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Alternative technique
polyclonals, rabbit-anti, murine, antibodies
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Alternative to gene target
tumor necrosis factor, DIF and TNF-alpha and TNFA and TNFSF2, TNF and IDBG-300259 and ENSG00000232810 and 7124, transcription regulatory region DNA binding, Extracellular, Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926, TNF and IDBG-634161 and ENSBTAG00000025471 and 280943
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