Rabbit Anti-Methylated Lysine Polyclonal: PE/ATTO 594
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Catalog numberSPC-158F-P594
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Price:
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Size400 µl
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ImmunogenMethylated KLH Conjugated
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Antibody s targetMethylated Lysine
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Antibody s full descriptionRabbit Anti-Methylated Lysine Polyclonal
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Primary research fieldsCell Signaling, Post-Translational Modifications, Methylation
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameDimethylysine Antibody, Methyl lysine Antibody, N epsilon dimethyl lysine Antibody, Trimethyl lysine Antibody
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Verified applicationsWB, ICC/IF, IP, ELISA
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Raised inRabbit
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Antibody s reactivitySpecies Independent
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Antibody s recommended dilutions for useWB (1:5000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromProtein A Purified
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Recommended buffer for storagePBS, 50% glycerol
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Antibody s concentration0.25 mg/ml
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Antibody s specificityDetects proteins containing methylated lysine residues.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysis0.2-0.5 µg/ml of SPC-158 was sufficient for detection of the methylated histone by western blot analysis using melanoma cells in TBSt.
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Antibody in cellContact our support service.
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextPost-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).
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Bibliography1. Yang X.J. (2005) Oncogene. 24:1653-1662. 2. Hassig C.A. and Schreiber S.L. (1997) Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang X.J. (2004) Bioessays 26:1076-1087. 4. Hughes R.E. (2002) Curr. Biol. 12: R141-R143. 5. Vigushin D.M. and Coombes R.C. (2004) Curr. Cancer Drug Targets 4: 205-218. 6. Chan H.M. et al. (2001) Nat. Cell Biol. 3: 667-674. 7. Martinez-Balbas M.A. et al. (2000) EMBO J. 19: 662-671.
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Released date1/Jun/2007
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NCBI numberRefer to NCBI
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Gene numberRefer to GenBank
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Protein numberRefer to Swiss-Prot
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PubMed number27858264|27521690|25609799|25374779|24651940|24233354|23533717
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Tested applicationsDot Blot|Western Blot|Western Blot|Western Blot|Dot Blot|Dot Blot|Dot Blot
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Tested reactivityOtala lactea (milk snail)|Squirrel|Hamster |Ictidomys tridecemlineatus (ground squirrel)|Xenopus laevis|Littorinalittorea (intertidal marine snail)|Turtle
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Methylated Lysine Antibody. (C) Composite. | Western blot analysis of Bovine serum albumin showing detection of Methylated Lysine protein using Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158). Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158) at 1:1000. Methylated Lysine in BSA (Left) and Methylated BSA (Right). Rabbit Anti-Methylated Lysine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-158) | Rabbit Anti-Methylated Lysine Antibody used in Western blot (WB) on Bovine serum albumin (SPC-158)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.4
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Short nameRabbit Anti-Methylated Lysine Polyclonal: PE/ATTO 594
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TechniquePolyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelPE/ATTO 594
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Alternative nameproduction species: rabbit Antibody toMethylated Lysine polyclonal: peroxidase/ATTO 594
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data