Rabbit Anti-KDEL Polyclonal: FITC
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Catalog numberSPC-109B-FITC
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Price:
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Size200 µg
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ImmunogenKDEL containing peptide immunogen
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Antibody s targetKDEL
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Antibody s full descriptionRabbit Anti-KDEL Polyclonal
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Primary research fieldsCell Signaling, Organelle Markers, Trafficking, Chaperones
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameLys Asp Glu Leu Antibody
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Verified applicationsWB, ICC/IF, IHC
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Raised inRabbit
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s recommended dilutions for useWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromProtein A Purified
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Recommended buffer for storagePBS pH7.2, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects KDEL proteins, GRP94, Grp78, PDI and calreticulin. It may also see ERp57 and ERp29.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-109 was sufficient for detection of KDEL-containing proteins in 20 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG as the secondary.
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Antibody in cellEndoplasmic Reticulum
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextThe endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
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Bibliography1. Ozawa K., et al. (2008) Mol Pharmacol. 74:1610. 2. Austin R.C., et al. (2003) J Biol Chem. 278: 17438.
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Released date16/May/2011
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NCBI numberRefer to NCBI
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Gene numberRefer to GenBank
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Protein numberRefer to Swiss-Prot
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-KDEL Polyclonal Antibody (SPC-109). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody (SPC-109) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-KDEL Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. | Western blot analysis of Human Cell line lysates showing detection of KDEL protein using Rabbit Anti-KDEL Polyclonal Antibody (SPC-109). Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody (SPC-109) at 1:1000, 1:500. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-KDEL Polyclonal Antibody (SPC-109). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody (SPC-109) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-KDEL Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. Rabbit Anti-KDEL Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-109) | Rabbit Anti-KDEL Antibody used in Western blot (WB) on Human Cell line lysates (SPC-109) | Rabbit Anti-KDEL Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-109)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.2
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesThis StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolKDELR3, POGLUT2, POGLUT2P1
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Short nameRabbit Anti-KDEL Polyclonal: FITC
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TechniquePolyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelFITC
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Alternative nameproduction species: rabbit Antibody toKDEL polyclonal: fluorecein
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Alternative techniquepolyclonals, rabbit-anti, antibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameKDEL endoplasmic reticulum protein retention receptor 3
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Synonyms gene name
- KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 3
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GenBank acession
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Locus
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Discovery year1999-08-19
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Entrez gene record
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Classification
- KDEL endoplasmic reticulum protein retention receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein O-glucosyltransferase 2
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Synonyms gene
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Synonyms gene name
- KDEL (Lys-Asp-Glu-Leu) containing 1
- KDEL motif containing 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-12-16
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Entrez gene record
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Pubmed identfication
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Classification
- Glycosyltransferase family 90
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namePOGLUT2 pseudogene 1
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Synonyms gene
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Synonyms gene name
- KDEL (Lys-Asp-Glu-Leu) containing 1 pseudogene 1
- KDEL motif containing 1 pseudogene 1
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Locus
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Discovery year2010-11-18
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Entrez gene record
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data