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Immunogen
Synthetic peptide of human VEGF Receptor 1 (AA143-158)
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Antibody s target
Human VEGF Receptor 1
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Antibody s full description
Rabbit Anti-Human VEGF Receptor 1 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
EC 2.7.10.1 Antibody, FLT 1 Antibody, FLT Antibody, Flt-1 Antibody, FLT1 Antibody, Fms like tyrosine kinase 1 Antibody, Fms related tyrosine kinase 1 Antibody, Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) Antibody, Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor Antibody, Fms-like tyrosine kinase 1 Antibody, FRT Antibody, Soluble VEGF receptor 1 14 Antibody, Soluble VEGFR1 variant 2 Antibody, Soluble VEGFR1 variant 21 Antibody, Tyrosine protein kinase FRT Antibody, Tyrosine protein kinase receptor FLT Antibody, Tyrosine-protein kinase FRT Antibody, Tyrosine-protein kinase receptor FLT Antibody, Vascular endothelial growth factor receptor 1 Antibody, Vascular endothelial growth factor vascular permeability factor receptor Antibody, Vascular permeability factor receptor 1 Antibody, Vascular permeability factor receptor Antibody, VEGFR 1 Antibody, VEGFR-1 Antibody, VEGFR1 Antibody, VGFR1_HUMAN Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 151 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1210 was sufficient for detection of VEGF Receptor 1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cell membrane, Secreted, Cytoplasm
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Tissue specificity
Detected in lung, placenta, liver, kidney, heart and brain tissues. Expressed in most vascular endothelial cells.
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Scientific context
FLT1 is a receptor-tyrosine kinase that is activated upon binding with VEGF-A, VEGF-B and PGF. It plays an important role in the development of embryonic vasculature, and as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. However, it can promote PGF-mediated endothelial cell proliferation, survival and angiogenesis in adults, although this seems to be cell-type specific. It also contributes to cancer cell survival, proliferation, migration, and invasion, as well as tumour angiogenesis, metastasis, and recruitment of tumour-infiltrating macrophages. It has a very high affinity for VEGF-A and may function as a negative regulator of VEGF-A signalling by limiting the amount of free VEGF-A and preventing its binding to KDR. Isoforms lacking a transmembrane domain may function as decoy receptors for VEGF-A. Furthermore, it mediates phosphorylation of PI3K, leading to activation of the downstream signalling pathway, as well as mediating the activation of the MAPK signalling pathway, and of the Akt/PKB signalling pathway.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_002010
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Gene number
2321
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Protein number
P17948
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
