Rabbit Anti-Human SP1 Polyclonal: ATTO 633

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• Catalog number
  SPC-1288D-A633
• Price:

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• Size
  100 µl

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• Immunogen
  A synthesized peptide derived from human SP1
• Antibody s target
  Human SP1
• Antibody s full description
  Rabbit Anti-Human SP1 Polyclonal
• Primary research fields
  Stem Cells, Signaling Pathways, TGF Beta, Nuclear, Cell Signaling, Metabolism, Nucleotide Metabolism
• Antibody s category
  Polyclonal Antibodies
• Antibody s other name
  TSFP1 Antibody, Transcription factor SP1 Antibody, SP 1 Antibody, Specificity protein 1 Antibody
• Verified applications
  WB, IHC, ICC/IF
• Raised in
  Rabbit
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s recommended dilutions for use
  WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
• Antibody s purified from
  Peptide Affinity Purified
• Recommended buffer for storage
  PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
• Antibody s concentration
  See included datasheet or contact our support service
• Antibody s specificity
  Detects ~81kDa, endogenous levels of total SP1
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Certificate of analysis
  A 1:1000 dilution of SPC-1288 was sufficient for detection of SP1 in 10 µg of HT29 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
• Antibody in cell
  Nucleus, Cytoplasm
• Tissue specificity
  Up-regulated in adenocarcinomas of the stomach (at protein level).
• Scientific context
  SP1 is a ubiquitously expressed transcription factor that belongs to the SP/KLF family. It is 785 amino acids long, with a molecular weight of 81 kDA. It interacts with many other transciption factors such as c-Myc, EGR1 and Stat1, and has also been used as a control protein to compare with when studying the increase or decrease of the aryl hyrdocarbon receptor and/or the estrogen receptor.Activity and stability are regulated by post-translational modifications. SP1 has been found to be high in patients with Alzheimer's diease.
• Bibliography
  1. Tan N.Y. and Khachigian L.M. (2009) Mol Cell Biol. 29: 2483-2488. 2. Citron B.A. et al. (2008) J Neurosci Res 86, 2499-504. 3. Wormke M., et al. (2003) Mol. Cell. Biol. 23(6): 1843–1855.
• Released date
  6/Sep/2016
• NCBI number
  NP_612482.2
• Gene number
  6667
• Protein number
  P08047
• PubMed number
  Refer to PubMed
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• Antibody s datasheet
  Contact our support service to receive datasheet or other technical documentation.
• Representative figure link
• Representative figure legend
  Western blot analysis of Human HT29 cell lysates showing detection of ~81kDa SP1 protein using Rabbit Anti-SP1 Polyclonal Antibody (SPC-1288). Lane 1: Human HT29 Whole Cell lysates treated with the immunizing peptide. Lane 2: Human HT29 Whole Cell lysates. Primary Antibody: Rabbit Anti-SP1 Polyclonal Antibody (SPC-1288) at 1:1000. Predicted/Observed Size: ~81kDa. Rabbit Anti-SP1 Antibody used in Western blot (WB) on HT29 cell lysates (SPC-1288)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availabilit
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Test
  StressMark antibodies supplies antibodies that are for research of human proteins.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

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• Gene target
  SP1   ATTO   633  
• Gene symbol
  SP1, PRSS55, ST14
• Short name
  Rabbit Anti- SP1 Polyclonal: ATTO 633
• Technique
  anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  IgG
• Label
  ATTO 633
• Species
  Human, Humans
• Alternative name
  production species: rabbit Antibody toHuman SP1 polyclonal: ATTO 633
• Alternative technique
  polyclonals, rabbit-anti, antibodies

Gene info

• Identity
  HGNC:11205
• Gene
  SP1
• Long gene name
  Sp1 transcription factor
• Synonyms name
  • specificity protein 1
• GenBank acession
  • J03133
• Locus
  12q13.13
• Discovery year
  1991-06-04
• Entrez gene record
  6667
• Pubmed identfication
  • 1662663
• Classification
  • Sp transcription factors
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000170047

Gene info

• Identity
  HGNC:30824
• Gene
  PRSS55
• Long gene name
  serine protease 55
• Synonyms gene name
  • protease, serine 55
• Synonyms
  • T-SP1
  • UNQ9391
  • CT153
• GenBank acession
  • AY358867
• Locus
  8p23.1
• Discovery year
  2010-04-14
• Entrez gene record
  203074
• Pubmed identfication
  • 12975309
  • 18844450
• RefSeq identity
  • NM_198464
• Classification
  • Serine proteases
• VEGA ID
  OTTHUMG00000129345

Gene info

• Identity
  HGNC:11344
• Gene
  ST14
• Long gene name
  ST14 transmembrane serine protease matriptase
• Synonyms gene
  • PRSS14
• Synonyms gene name
  • suppression of tumorigenicity 14 (colon carcinoma)
  • suppression of tumorigenicity 14
• Synonyms
  • SNC19
  • HAI
  • MT-SP1
  • TMPRSS14
  • CAP3
• Synonyms name
  • epithin
  • matriptase
  • channel–activating protein 3
• GenBank acession
  • AF118224
• Locus
  11q24.3
• Discovery year
  1998-05-05
• Entrez gene record
  6768
• Pubmed identfication
  • 9925927
  • 10373424
• Classification
  • Type II transmembrane serine proteases
• VEGA ID
  OTTHUMG00000165768

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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