Rabbit Anti-Human SP1 Polyclonal: ATTO 488
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Catalog numberSPC-1288D-A488
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Price:
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Size100 µl
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ImmunogenA synthesized peptide derived from human SP1
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Antibody s targetHuman SP1
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Antibody s full descriptionRabbit Anti-Human SP1 Polyclonal
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Primary research fieldsStem Cells, Signaling Pathways, TGF Beta, Nuclear, Cell Signaling, Metabolism, Nucleotide Metabolism
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameTSFP1 Antibody, Transcription factor SP1 Antibody, SP 1 Antibody, Specificity protein 1 Antibody
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Verified applicationsWB, IHC, ICC/IF
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Raised inRabbit
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s recommended dilutions for useWB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects ~81kDa, endogenous levels of total SP1
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-1288 was sufficient for detection of SP1 in 10 µg of HT29 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cellNucleus, Cytoplasm
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Tissue specificityUp-regulated in adenocarcinomas of the stomach (at protein level).
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Scientific contextSP1 is a ubiquitously expressed transcription factor that belongs to the SP/KLF family. It is 785 amino acids long, with a molecular weight of 81 kDA. It interacts with many other transciption factors such as c-Myc, EGR1 and Stat1, and has also been used as a control protein to compare with when studying the increase or decrease of the aryl hyrdocarbon receptor and/or the estrogen receptor.Activity and stability are regulated by post-translational modifications. SP1 has been found to be high in patients with Alzheimer's diease.
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Bibliography1. Tan N.Y. and Khachigian L.M. (2009) Mol Cell Biol. 29: 2483-2488. 2. Citron B.A. et al. (2008) J Neurosci Res 86, 2499-504. 3. Wormke M., et al. (2003) Mol. Cell. Biol. 23(6): 1843–1855.
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Released date6/Sep/2016
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NCBI numberNP_612482.2
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Gene number6667
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Protein numberP08047
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure legendWestern blot analysis of Human HT29 cell lysates showing detection of ~81kDa SP1 protein using Rabbit Anti-SP1 Polyclonal Antibody (SPC-1288). Lane 1: Human HT29 Whole Cell lysates treated with the immunizing peptide. Lane 2: Human HT29 Whole Cell lysates. Primary Antibody: Rabbit Anti-SP1 Polyclonal Antibody (SPC-1288) at 1:1000. Predicted/Observed Size: ~81kDa. Rabbit Anti-SP1 Antibody used in Western blot (WB) on HT29 cell lysates (SPC-1288)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolSP1, PRSS55, ST14
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Short nameRabbit Anti- SP1 Polyclonal: ATTO 488
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeIgG
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LabelATTO 488
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman SP1 polyclonal: ATTO 488
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameSp1 transcription factor
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-06-04
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Entrez gene record
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Pubmed identfication
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Classification
- Sp transcription factors
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameserine protease 55
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Synonyms gene name
- protease, serine 55
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Synonyms
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GenBank acession
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Locus
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Discovery year2010-04-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Serine proteases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameST14 transmembrane serine protease matriptase
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Synonyms gene
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Synonyms gene name
- suppression of tumorigenicity 14 (colon carcinoma)
- suppression of tumorigenicity 14
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-05-05
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Entrez gene record
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Pubmed identfication
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Classification
- Type II transmembrane serine proteases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data