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Immunogen
Human Mn SOD
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Antibody s target
Human SOD2
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Antibody s full description
Rabbit Anti-Human SOD (Mn) Polyclonal
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Primary research fields
Cancer, Oxidative Stress, Cell Signaling, Neuroscience, Neurodegeneration, Cardiovascular System
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Manganese SOD Antibody, IPO B Antibody, Mn SOD Antibody, SOD2 Antibody
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Verified applications
WB, IHC, IP, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Rat, Mouse, Bovine, Dog, Chicken, Gerbil, Guinea Pig (Cavia porcellus), Pig, Hamster, Monkey, Rabbit, Sheep, African clawed frog (Xenopus laevis), Frog, Wood Frog (Rana sylvatica)
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Antibody s recommended dilutions for use
WB (1:5000), IHC (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~25kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
0.2 µg/ml of SPC-118 was sufficient for detection of Mn SOD in 20 µg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-mouse IgG:AP as the secondary antibody.
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Antibody in cell
Mitochondrion, Mitochondrion Matrix
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).
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Bibliography
1. Adachi T., et al. (1992). Clin. Chim. Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. Furukawa Y., O’Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6. 4. Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe N., et al. (1990) Biomedical Research. 11: 187-194 7. Wispe J.R., et al. (1989) BBA. 994: 30-36. 8. Xiao-Hong Liu., et al. (1993) Brain Research. 625: 29-37.
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Released date
1/Jun/2007
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NCBI number
NP_000627.2
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Gene number
6648
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Protein number
P04179
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PubMed number
25316288
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Tested applications
Western Blot
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Tested reactivity
North American wood frog (Rana sylvatica)
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-SOD2 Polyclonal Antibody (SPC-118). Tissue: muscle fibres. Species: Rat. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody (SPC-118) at 1:100. Left: Untreated, Right: treated with 3mmol*kg-1 NAC. Courtesy of: E. Barreiro, IMIM, Spain.. Rabbit Anti-SOD2 Antibody used in Immunohistochemistry (IHC) on Rat muscle fibres (SPC-118)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
