Rabbit Anti-Human Smad4 Polyclonal: ATTO 633
#
-
Catalog numberSPC-1286D-A633
-
Price:
-
Size100 µl
-
-
ImmunogenSynthesized peptide derived from internal of human Smad4.
-
Antibody s targetHuman Smad4
-
Antibody s full descriptionRabbit Anti-Human Smad4 Polyclonal
-
Primary research fieldsCell Signaling, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic, Cardiovascular System, Heart, Cardiogenesis, Cell Signaling, Metabolism, Metabolism processes, Hypoxia
-
Antibody s categoryPolyclonal Antibodies
-
Antibody s other name(Small) Mothers Against Decapentaplegic Antibody, Deleted in Pancreatic Carcinoma 4 Antibody, DPC 4 Antibody, hSMAD4 Antibody, JIP Antibody, MADH 4 Antibody, Mothers against decapentaplegic homolog 4 Antibody, SMAD 4 Antibody, SMAD mothers against DPP homolog 4 Antibody
-
Verified applicationsWB, ELISA
-
Raised inRabbit
-
Antibody s reactivityHuman, Mouse, Rat
-
Antibody s recommended dilutions for useWB (1:1000), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
-
Antibody s purified fromPeptide Affinity Purified
-
Recommended buffer for storagePBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
-
Antibody s concentrationSee included datasheet or contact our support service
-
Antibody s specificityDetects ~60kDa, endogenous levels of total Smad4 protein.
-
Storage recommendations-20°C
-
Shipping recommendationsBlue Ice or 4°C
-
Certificate of analysisA 1:1000 dilution of SPC-1286 was sufficient for detection of Smad4 in 10 µg of HeLa, Cos7, HepG2, HUVEC cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
-
Antibody in cellCytoplasm, Nucleus
-
Tissue specificitySee included datasheet or contact our support service.
-
Scientific contextMembers of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Smad4 mediates all activities through interaction with various other Smad family members.
-
Bibliography1. Heldin C.H. et al. (1997) Nature. 390: 465-71. 2. Attisano L. and Wrana, J.L. (1998) Curr Opin Cell Biol. 10: 188-94. 3. Massagué J. (1998) Annu Rev Biochem. 67: 753-91. 4. Whitman M. (1998) Genes Dev. 12: 2445-62. 5. Wu G. et al. (2000) Science. 287: 92-7.
-
Released date6/Sep/2016
-
NCBI numberNP_005350.1
-
Gene number4089
-
Protein numberQ13485
-
PubMed numberRefer to PubMed
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
-
Representative figure legendWestern blot analysis of Human, Monkey HeLa, Cos7, HepG2, HUVEC cell lysates showing detection of ~60kDa Smad4 protein using Rabbit Anti-Smad4 Polyclonal Antibody (SPC-1286). Lane 1: Human HeLa. Lane 2: Monkey Cos7. Lane 3: Human HepG2. Lane 4: Human HUVEC. Lane 5: Treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Smad4 Polyclonal Antibody (SPC-1286) at 1:1000. Predicted/Observed Size: ~60kDa. Rabbit Anti-Smad4 Antibody used in Western blot (WB) on HeLa, Cos7, HepG2, HUVEC cell lysates (SPC-1286)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilitIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
TestStressMark antibodies supplies antibodies that are for research of human proteins.
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
GroupPolyclonals and antibodies
-
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin nameOryctolagus cuniculus
-
Gene target
-
Short nameRabbit Anti- Smad4 Polyclonal: ATTO 633
-
Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
HostRabbit, Rabbits
-
IsotypeIgG
-
LabelATTO 633
-
SpeciesHuman, Humans
-
Alternative nameproduction species: rabbit Antibody toHuman SMAD family member 4 polyclonal: ATTO 633
-
Alternative techniquepolyclonals, rabbit-anti, antibodies
-
Alternative to gene targetSMAD family member 4, DPC4 and JIP and MADH4 and MYHRS, SMAD4 and IDBG-3485 and ENSG00000141646 and 4089, transforming growth factor beta receptor, nuclei, Smad4 and IDBG-157503 and ENSMUSG00000024515 and 17128, SMAD4 and IDBG-644730 and ENSBTAG00000006919 and 540248
-
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data