Rabbit Anti-Human Piccolo Polyclonal: ATTO 655
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Catalog numberSPC-197D-A655
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Price:
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Size100 µg
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ImmunogenFull length protein, BC001304.1
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Antibody s targetHuman Piccolo
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Antibody s full descriptionRabbit Anti-Human Piccolo Polyclonal
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Primary research fieldsNeuroscience, Cell Structure, Pre-Synaptic Markers
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameACZ Antibody, Aczonin Antibody, Brain-derived HLMN protein Antibody, DKFZp779G1236 Antibody, KIAA0559 Antibody, Multidomain presynaptic cytomatrix protein Antibody, PCLO Antibody, PCLO_HUMAN Antibody, Piccolo (presynaptic cytomatrix protein) Antibody, Piccolo mouse homolog of Pico Antibody, Presynaptic cytomatrix protein Antibody, Protein piccolo Antibody
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Verified applicationsWB, IHC
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Raised inRabbit
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s recommended dilutions for useWB (1:1000), IHC (1:400); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromProtein A Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~550kDa. No cross-reactivity to Bassoon.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysis1 µg/ml of SPC-197 was sufficient for detection of Piccolo in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCell Junction, Synapse
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextPiccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).
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Bibliography1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.
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Released date22/Mar/2011
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NCBI numberNP_055325.2
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Gene number27445
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Protein numberQ9Y6VO
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197). Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197) at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ. | Western blot analysis of Mouse, Rat brain cell lysates showing detection of Piccolo protein using Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197). Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197) at 1:1000. Rabbit Anti-Piccolo Antibody used in Immunohistochemistry (IHC) on Mouse Muscle (SPC-197) | Rabbit Anti-Piccolo Antibody used in Western blot (WB) on Mouse, Rat brain cell lysates (SPC-197)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Short nameRabbit Anti- Piccolo Polyclonal: ATTO 655
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelATTO 655
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman Piccolo polyclonal: ATTO 655
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data