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Immunogen
A 20 residue synthetic peptide based on the human p38 with the cysteine residue added and coupled to KLH
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Antibody s target
Human p38
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Antibody s full description
Rabbit Anti-Human p38 Polyclonal
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Primary research fields
Cell Signaling, Post-translational modifications, Phosphorylation, Cancer
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
CSAID Binding protein 1 Antibody, CSBP1 Antibody, CSBP2 Antibody, EXIP Antibody, MAP kinase MXI2 Antibody, MAPkinase p38alpha Antibody, MAPK14 Antibody, p38 ALPHA Antibody, p38 MAP kinase Antibody, p38 mitogen activated protein kinase Antibody, RK Antibody, SAPK 2A Antibody, Stress activated protein kinase 2A Antibody
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Verified applications
WB, IHC, ICC/IF, IP
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat, Bovine, Monkey, Rabbit, Pig, Dog, Hamster, Chicken, Sheep, Guinea Pig (Cavia porcellus)
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Antibody s recommended dilutions for use
WB (1:1000), ICC/IF (1:100), IP (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~43kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-172 was sufficient for detection of p38 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
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Antibody in cell
Cytoplasm, Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, protein-serine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).
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Bibliography
1. Pearson G., et al (2001) Endocrine Reviews 22 (2): 153-183. 2. Fan Y., et al (2007) Mol. Cells 23 (1): 30-38. 3. Han J., et al. (1994) Science 265: 808-811. 4. Van L. A., et al. (2004) Faseb J. 18: 1946−1948. 5. Deng et al. (2003) Cell 115: 61-70. 6. Salojin K.V., et al. (2006) J Immunol. 176 (3):1899-907. 7. Medicherla S., et al. (2006) J Pharmacol Exp Ther. 318(1): 99-107.
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Released date
1/May/2008
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NCBI number
NP_001306.1
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Gene number
1432
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Protein number
Q16539
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PubMed number
27333444
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Tested applications
Western Blot
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Tested reactivity
Mouse
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p38 Polyclonal Antibody (SPC-172). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody (SPC-172) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p38 Antibody. (C) Composite. | Western blot analysis of Human cancer cell lines showing detection of p38 protein using Rabbit Anti-p38 Polyclonal Antibody (SPC-172). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody (SPC-172) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p38 Polyclonal Antibody (SPC-172). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody (SPC-172) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-p38 Antibody. (C) Composite. Rabbit Anti-p38 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-172) | Rabbit Anti-p38 Antibody used in Western blot (WB) on Human cancer cell lines (SPC-172) | Rabbit Anti-p38 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-172)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
