Rabbit Anti-Human p38 Polyclonal: FITC
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Catalog numberSPC-172D-FITC
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Price:
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Size100 µl
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ImmunogenA 20 residue synthetic peptide based on the human p38 with the cysteine residue added and coupled to KLH
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Antibody s targetHuman p38
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Antibody s full descriptionRabbit Anti-Human p38 Polyclonal
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Primary research fieldsCell Signaling, Post-translational modifications, Phosphorylation, Cancer
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameCSAID Binding protein 1 Antibody, CSBP1 Antibody, CSBP2 Antibody, EXIP Antibody, MAP kinase MXI2 Antibody, MAPkinase p38alpha Antibody, MAPK14 Antibody, p38 ALPHA Antibody, p38 MAP kinase Antibody, p38 mitogen activated protein kinase Antibody, RK Antibody, SAPK 2A Antibody, Stress activated protein kinase 2A Antibody
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Verified applicationsWB, IHC, ICC/IF, IP
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Raised inRabbit
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Antibody s reactivityHuman, Mouse, Rat, Bovine, Monkey, Rabbit, Pig, Dog, Hamster, Chicken, Sheep, Guinea Pig (Cavia porcellus)
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Antibody s recommended dilutions for useWB (1:1000), ICC/IF (1:100), IP (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~43kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-172 was sufficient for detection of p38 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
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Antibody in cellCytoplasm, Nucleus
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextThe MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, protein-serine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).
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Bibliography1. Pearson G., et al (2001) Endocrine Reviews 22 (2): 153-183. 2. Fan Y., et al (2007) Mol. Cells 23 (1): 30-38. 3. Han J., et al. (1994) Science 265: 808-811. 4. Van L. A., et al. (2004) Faseb J. 18: 1946−1948. 5. Deng et al. (2003) Cell 115: 61-70. 6. Salojin K.V., et al. (2006) J Immunol. 176 (3):1899-907. 7. Medicherla S., et al. (2006) J Pharmacol Exp Ther. 318(1): 99-107.
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Released date1/May/2008
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NCBI numberNP_001306.1
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Gene number1432
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Protein numberQ16539
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PubMed number27333444
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Tested applicationsWestern Blot
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Tested reactivityMouse
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p38 Polyclonal Antibody (SPC-172). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody (SPC-172) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p38 Antibody. (C) Composite. | Western blot analysis of Human cancer cell lines showing detection of p38 protein using Rabbit Anti-p38 Polyclonal Antibody (SPC-172). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody (SPC-172) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p38 Polyclonal Antibody (SPC-172). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody (SPC-172) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-p38 Antibody. (C) Composite. Rabbit Anti-p38 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-172) | Rabbit Anti-p38 Antibody used in Western blot (WB) on Human cancer cell lines (SPC-172) | Rabbit Anti-p38 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-172)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesThis StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAnti-FITC Antibody
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolMAPK14, AIMP2, RPP38
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Short nameRabbit Anti- p38 Polyclonal: FITC
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelFITC
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman p38 polyclonal: fluorecein
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Alternative techniquepolyclonals, rabbit-anti, antibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene namemitogen-activated protein kinase 14
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-01-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitogen-activated protein kinases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameaminoacyl tRNA synthetase complex interacting multifunctional protein 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2009-05-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameribonuclease P/MRP subunit p38
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Synonyms gene name
- ribonuclease P/MRP 38kDa subunit
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GenBank acession
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Locus
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Discovery year2004-03-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data