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Immunogen
Full length human GRP78 (Bip) his tagged at the N terminus
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Antibody s target
Human GRP78
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Antibody s full description
Rabbit Anti-Human GRP78 (Bip) Polyclonal
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Primary research fields
Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones, Organelle Markers
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
BIP Antibody, Grp78 Antibody, HSPA5 Antibody, MIF2 Antibody, immunoglobulin heavy chain binding protein Antibody
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Verified applications
WB, IHC, ICC/IF, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat, Dog, Fruit Fly (Drosophila melanogaster)
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Antibody s recommended dilutions for use
WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~78kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
0.5 µg/ml of SPC-180 was sufficient for detection of Grp78 in 10 µg of rat tissue lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Endoplasmic Reticulum, Endoplasmic Reticulum Membrane, Melanosome
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
GRP78 is a ubiquitously expressed, 78-kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
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Bibliography
1. Cho S., et al. (2007) Mol Cell Biol. 27(1): 368-83. 2. Yang Y., et al. (1998) J Biol Chem. 273: 25552-25555. 3. Luo S., et al (2006) 26 (15): 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19: 4333-4336. 6. Laquerre S., et al. (1998) J. Virology. 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res. 65(13): 5785-91.
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Released date
23/Mar/2011
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NCBI number
NP_005338.1
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Gene number
3309
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Protein number
P11021
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PubMed number
24801175|24183663
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Tested applications
Western Blot|Immunohistochemistry
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Tested reactivity
Mouse |Drosophila
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 (Bip) Polyclonal Antibody (SPC-180). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 (Bip) Polyclonal Antibody (SPC-180) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 (Bip) Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of Human Glucose deprived glia cell lysates showing detection of GRP78 protein using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-180). Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-180) at 1:1000. Rabbit Anti-GRP78 (Bip) Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-180) | Rabbit Anti-GRP78 Antibody used in Western blot (WB) on Human Glucose deprived glia cell lysates (SPC-180)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
