Rabbit Anti-Human GLUT1 Polyclonal: ATTO 390
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Catalog numberSPC-1295D-A390
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Price:
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Size100 µl
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ImmunogenSynthesized peptide derived from human GLUT1.
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Antibody s targetHuman GLUT1
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Antibody s full descriptionRabbit Anti-Human GLUT1 Polyclonal
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Primary research fieldsCancer, Cardiovascular System, Atherosclerosis
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameChoreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) Antibody, CSE Antibody, DYT17 Antibody, DYT18 Antibody, DYT9 Antibody, EIG12 Antibody, erythrocyte/brain Antibody, Erythrocyte/hepatoma glucose transporter Antibody, facilitated glucose transporter member 1 Antibody, Glucose transporter 1 Antibody, Glucose transporter type 1 Antibody, Glucose transporter type 1, erythrocyte/brain Antibody, GLUT Antibody, GLUT-1 Antibody, GLUT1 Antibody, GLUT1DS Antibody, GLUTB Antibody, GT1 Antibody, GTG1 Antibody, Gtg3 Antibody, GTR1_HUMAN Antibody, HepG2 glucose transporter Antibody, HTLVR Antibody, Human T cell leukemia virus (I and II) receptor Antibody, MGC141895 Antibody, MGC141896 Antibody, PED Antibody, RATGTG1 Antibody, Receptor for HTLV 1 and HTLV 2 Antibody, SLC2A1 Antibody, Solute carrier family 2 (facilitated glucose transporter), member 1 Antibody, Solute carrier family 2 Antibody, Solute carrier family 2, facilitated glucose transporter member 1 Antibody
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Verified applicationsWB, IHC, ELISA
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Raised inRabbit
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s recommended dilutions for useWB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:40000); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects ~54kDa, endogenous levels of total GLUT1 protein.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1000 dilution of SPC-1295 was sufficient for detection of GLUT1 in 10 µg of Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane, Melanosome
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Tissue specificityDetected in erythrocytes (at protein level). Expressed at variable levels in many human tissues.
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Scientific contextGlucose transporter 1 is a uniporter protein that in humans is encoded by the SLC2A1 gene, also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1). GLUT-1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55kDa and a 45 kDa. The 55kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier, whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake.
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Bibliography1. Mueckler M. et al. (1985) Science 229 (4717): 941–5. 2. Olson A.L. and Pessin J.E. (1996) Annual Review of Nutrition 16: 235–56.
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Released date6/Sep/2016
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NCBI numberNP_006507.2
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Gene number6513
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Protein numberP11166
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295). Tissue: Brain Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human Jurkat cell lysates showing detection of ~55kDa GLUT1 protein using Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295). Lane 1: Human Jurkat cells. Lane 2: Human Jurkat cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295) at 1:1000. Predicted/Observed Size: ~55kDa. Rabbit Anti-GLUT1 Antibody used in Immunohistochemistry (IHC) on Brain Tissue (SPC-1295) | Rabbit Anti-GLUT1 Antibody used in Western blot (WB) on Jurkat cell lysates (SPC-1295)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolSLC2A1
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Short nameRabbit Anti- GLUT1 Polyclonal: ATTO 390
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeIgG
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LabelATTO 390
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman GLUT1 polyclonal: ATTO 390
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene namesolute carrier family 2 member 1
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Synonyms gene
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Synonyms gene name
- human T-cell leukemia virus (I and II) receptor
- choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity)
- solute carrier family 2 (facilitated glucose transporter), member 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-11-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Solute carriers
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data