Rabbit Anti-Human Collagen II Polyclonal: ATTO 565
#
-
Catalog numberSPC-1274D-A565
-
Price:
-
Size100 µl
-
-
ImmunogenSynthesized peptide derived from human Collagen II.
-
Antibody s targetHuman Collagen II
-
Antibody s full descriptionRabbit Anti-Human Collagen II Polyclonal
-
Primary research fieldsCell Signaling, Extracellular Matrix (ECM), Collagen, Stem Cells, Mesenchymal Stem Cells, Osteogenesis, Cell Signaling, Extracellular Matrix (ECM), Bone
-
Antibody s categoryPolyclonal Antibodies
-
Antibody s other nameAlpha 1 type II collagen Antibody, AOM Antibody, Cartilage collagen Antibody, Chondrocalcin Antibody, COL11A3 Antibody, Collagen II alpha 1 polypeptide Antibody, SEDC Antibody
-
Verified applicationsWB, IHC, ICC/IF, ELISA
-
Raised inRabbit
-
Antibody s reactivityHuman, Mouse, Rat
-
Antibody s recommended dilutions for useWB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
-
Antibody s purified fromPeptide Affinity Purified
-
Recommended buffer for storagePBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
-
Antibody s concentrationSee included datasheet or contact our support service
-
Antibody s specificityDetects endogenous levels of total Collagen II protein.
-
Storage recommendations-20°C
-
Shipping recommendationsBlue Ice or 4°C
-
Certificate of analysisA 1:1000 dilution of SPC-1274 was sufficient for detection of Collagen II in 10 µg of COLO205 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
-
Antibody in cellExtracellular matrix
-
Tissue specificityIsoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.
-
Scientific contextType-II collagen is the less abundant form of collagen in the human body and can be found in articular and hyaline cartilages and the eyes. It has been associated with the treatment of arthritis, cellulite and wrinkles.
-
Bibliography1. Mullazehi M., et al. (2012) Arthritis Research Ther. 14:R100
-
Released date6/Sep/2016
-
NCBI numberNP_149162.2
-
Gene number1280
-
Protein numberP02458
-
PubMed numberRefer to PubMed
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
-
Representative figure link
-
Representative figure legendImmunohistochemistry analysis using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Tissue: Cos7. Species: Monkey. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human COLO205 cell lysates showing detection of ~142kDa Collagen II protein using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Lane 1: Human COLO205. Lane 2: Human COLO205 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:1000. Predicted/Observed Size: ~142kDa. Rabbit Anti-Collagen II Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1274) | Rabbit Anti-Collagen II Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cos7 (SPC-1274) | Rabbit Anti-Collagen II Antibody used in Western blot (WB) on COLO205 cell lysates (SPC-1274)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilitIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
TestStressMark antibodies supplies antibodies that are for research of human proteins.
-
GeneCollagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
GroupPolyclonals and antibodies
-
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin nameOryctolagus cuniculus
-
Gene target
-
Short nameRabbit Anti- Collagen II Polyclonal: ATTO 565
-
Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
HostRabbit, Rabbits
-
IsotypeIgG
-
LabelATTO 565
-
SpeciesHuman, Humans
-
Alternative nameproduction species: rabbit Antibody toHuman Collagen II polyclonal: ATTO 565
-
Alternative techniquepolyclonals, rabbit-anti, antibodies
-
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data