-
Immunogen
Native human Cdc37, full length
-
Antibody s target
Human CDC37
-
Antibody s full description
Rabbit Anti-Human CDC37 Polyclonal
-
Primary research fields
Cancer, Heat Shock, Cell Signaling, Epigenetics
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
Hsp90 co chaperone Cdc37 antibody, CDC 37 antibody, Cdc37 antibody, CDC37 cell division cycle 37 homolog antibody, CDC37 cell division cycle 37 S cerevisiae homolog antibody, CDC37 cell division cycle 37 S cerevisiae homolog of antibody, Cdc37 homolog antibody, CDC37 protein antibody, CDC37_HUMAN antibody, CDC37A antibody, cell division cycle 37 antibody, Cell division cycle 37 homolog antibody, Hsp90 chaperone protein kinase targeting subunit antibody, Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody, Hsp90 chaperone protein kinase-targeting subunit antibody, Hsp90 co-chaperone Cdc37 antibody, p50 antibody, p50Cdc37 antibody, S cerevisiae hypothetical protein CDC37 antibody
-
Verified applications
WB, ICC/IF
-
Raised in
Rabbit
-
Antibody s reactivity
Human
-
Antibody s recommended dilutions for use
WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Protein A Purified
-
Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration
1.68 mg/ml
-
Antibody s specificity
Detects ~44.5kDa.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:2000 dilution of SPC-142 was sufficient for detection of cdc37 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
-
Antibody in cell
Cytoplasm
-
Tissue specificity
See included datasheet or contact our support service.
-
Scientific context
HSP90 co-chaperone Cdc37 is a protein that is encoded by the CDC37 gene. It has been found to form complexes with HSP90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK and elF-2 alpha kinases. It is thought to play a critical role in directing HSP90 to its target kinases (1, 2). CDC37 is necessary for maintaining prostate tumor cell growth and represents a novel target in the exploration for multi-targeted therapies (3, 4).
-
Bibliography
1. Dai K, Kobayashi R., Beach D. (1996) J Biol Chem. 271(36): 22030-22034. 2. Stepanova L, Leng X., Parker S.B., Harper J.W. (1996) Genes Dev. 10(12): 1491-1502. 3. Kimura Y., et al. (1997) Genes Dev. 11(14): 1775-1185. 4. Gray P.J., et al. (2008) Nat Rev Cancer. 8(7): 491-495.
-
Released date
1/Jan/2012
-
NCBI number
NP_008996.1
-
Gene number
11140
-
Protein number
Q16543
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
Representative figure link
-
Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-CDC37 Polyclonal Antibody (SPC-142). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody (SPC-142) at 1:200 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-CDC37 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. | Western blot analysis of Human HeLa cell lysates showing detection of CDC37 protein using Rabbit Anti-CDC37 Polyclonal Antibody (SPC-142). Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody (SPC-142) at 1:2000. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-CDC37 Polyclonal Antibody (SPC-142). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody (SPC-142) at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-CDC37 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. Rabbit Anti-CDC37 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-142) | Rabbit Anti-CDC37 Antibody used in Western blot (WB) on Human HeLa cell lysates (SPC-142) | Rabbit Anti-CDC37 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-142)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
Gene target
-
Gene symbol
CDC37
-
Short name
Rabbit Anti- CDC37 Polyclonal: PE/ATTO 594
-
Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit, Rabbits
-
Isotype
Immunoglobulin
-
Label
PE/ATTO 594
-
Species
Human, Humans
-
Alternative name
production species: rabbit Antibody toHuman cellular division cycle 37 homolog (S. cerevisiae) polyclonal: peroxidase/ATTO 594
-
Alternative technique
polyclonals, rabbit-anti, antibodies
-
Alternative to gene target
cell division cycle 37 homolog (S. cerevisiae), P50CDC37, CDC37 and IDBG-27267 and ENSG00000105401 and 11140, Hsp90 protein binding, Cytoplasm, Cdc37 and IDBG-139272 and ENSMUSG00000019471 and 12539, CDC37 and IDBG-643270 and ENSBTAG00000011699 and
-