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Immunogen
Human Calcineurin A peptide (AA 264-283)
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Antibody s target
Human Calcineurin A
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Antibody s full description
Rabbit Anti-Human Calcineurin A Polyclonal
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Primary research fields
Cell Signaling, Post-translational modifications, Phosphorylation
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Alpha isoform formerly PPP2B Antibody, Calcineurin A alpha Antibody, Calcineurin A1 Antibody, CalcineurinA Antibody, Calmodulin dependent calcineurin A subunit alpha isoform Antibody, Calmodulin-dependent calcineurin A subunit alpha isoform Antibody, CALN Antibody, CALNA Antibody, CALNA 1 Antibody, CAM PRP catalytic subunit Antibody, CCN 1 Antibody, Protein phosphatase 2B catalytic subunitProtein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform Antibody, Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA Antibody, Protein phosphatase 3 catalytic subunit alpha isozyme Antibody, Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform Antibody
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Verified applications
WB, IHC, ICC/IF, IP
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat, Dog, Rabbit
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Antibody s recommended dilutions for use
WB (1:1000), ICC/IF (1:120), IHC (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~61kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
1 µg/ml of SPC-175 was sufficient for detection of Calcineurin A in 20 µg of Heat shock Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Calcineurin is a heterodimeric phosphatase protein, also known as calcium-dependent serine-threonine phosphatase. The structure consists of a catalytic subunit alpha, Calcineurin A (57-59KDa) the active site and a Ca2+ binding unit, Calcineurin B (19-20KDa) the regulatory subunit(1). Calcineurin plays a key role in the T-cell response growth and differentiation mechanism, regulating the activation of the Nuclear factor of activated T-cells (NFATc) which are important in the expression of IL-2 genes. Calcineurin has been the target of inhibitors, the novel and structural immune-suppressants antifungal drugs(2). Genetic studies in yeast and fungi established the molecular basis of the inhibition mechanism by cyclosporine A and FK506 (3).
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Bibliography
1. Rusnak F. and Mertz P. (2000) Physiol Rev. 80(4):1483-521. 2. Hemenway C.S. And Heitman J. (1999)Cell Biochem Biophys. 30(1):115-51. 3. Fox D.S. and Heitman J. (2002) Bioessays. 24(10):894-903.
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Released date
9/May/2013
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NCBI number
Q08209
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Gene number
5530
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Protein number
Q08209
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calcineurin A Polyclonal Antibody (SPC-175). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calcineurin A Polyclonal Antibody (SPC-175) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Calcineurin A Antibody. (C) Composite. | Immunohistochemistry analysis using Rabbit Anti-Calcineurin A Polyclonal Antibody (SPC-175). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calcineurin A Polyclonal Antibody (SPC-175) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: General epidermis. | Western blot analysis of Rat Brain cell lysates showing detection of ~61 kDa Calcineurin A protein using Rabbit Anti-Calcineurin A Polyclonal Antibody (SPC-175). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Calcineurin A Polyclonal Antibody (SPC-175) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~61 kDa. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calcineurin A Polyclonal Antibody (SPC-175). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calcineurin A Polyclonal Antibody (SPC-175) at 1:120 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Calcineurin A Antibody. (C) Composite. Rabbit Anti-Calcineurin A Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-175) | Rabbit Anti-Calcineurin A Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-175) | Rabbit Anti-Calcineurin A Antibody used in Western blot (WB) on Brain cell lysates (SPC-175) | Rabbit Anti-Calcineurin A Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-175)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
