Mouse Anti-Yeast HDEL Monoclonal IgG2b, Clone 2E7
#
-
Catalog numberSMC-175D-A565
-
Price:
-
Size100 µg
-
-
Clone2E7
-
ImmunogenRaised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
-
Antibody s full descriptionMouse Anti-Yeast HDEL Monoclonal IgG2b Antibody, Clone: 2E7: ATTO 565
-
Antibody s categoryMonoclonal Antibodies
-
Antibody s other nameH-D-E-L (his-asp-glu-leu) Antibody, endoplasmic reticulum Antibody, luminal ER protein retention Antibody, KDELR1 Antibody, Endoplasmic reticulum retention signal Antibody
-
Raised inMouse
-
Antibody s targetHDEL
-
Primary research fieldsCancer, Heat Shock, Cell Signaling, Trafficking
-
BrandnameNone
-
Antibodies applicationsWB, ICC/IF
-
Antibody s reactivityFruit Fly (Drosophila melanogaster), Yeast, Yeast (Saccharomyces cerevisiae), Plant, Barnyard Grass (Echinochloa), Beet (Beta vulgaris), Cotton (Gossypium), Mung Bean (Vigna radiata), Grass (Sorghum), Wheat (Triticum spp.)
-
Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
-
PurityProtein G Purified
-
Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration1 mg/ml
-
Antibody s specificityDetects ~78kDa.
-
Storage recommendations-20ºC
-
Shipping recommendationsBlue Ice or 4ºC
-
Antibody certificate of analysis1 µg/ml of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cellEndoplasmic Reticulum
-
Tissue specificitySee included datasheet or contact our support service
-
Scientific contextHSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
-
Bibliography1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62(6): 670-684. 2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26(15): 5688-5697. 3. Entrez Gene: HDEL, Gene ID: 10945 4. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271.
-
Released date1-Apr-2010
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
NCBI numberRefer to NCBI
-
Gene numberRefer to GenBank
-
Protein numberRefer to Swiss-Prot
-
Antibody s datasheetContact our support service
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilityIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus
-
Gene target
-
Gene symbolKDELR1
-
Short nameMouse Anti-Yeast HDEL Monoclonal IgG2b, Clone 2E7
-
Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
-
Hostmouse, Ascomycota
-
IsotypeIgG2b, IgG2b
-
LabelATTO 565
-
SpeciesYeast, Mouses, Yeasts
-
Alternative nameMouse Antibody toYeast HDEL monoclonal IgG2b, clonality 2E7
-
Alternative techniquemurine, antibodies
-
Clone name2E7
-
Gene info
-
Identity
-
Gene
-
Long gene nameKDEL endoplasmic reticulum protein retention receptor 1
-
Synonyms gene name
- KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1999-08-20
-
Entrez gene record
-
Pubmed identfication
-
Classification
- KDEL endoplasmic reticulum protein retention receptors
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data