Mouse Anti-Rat TrpC4 Monoclonal IgG1, Clone S77-15
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Catalog numberSMC-315D-BI
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PricePlease ask
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Size100 µg
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CloneS77-15
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ImmunogenSynthetic peptide amino acids 930-947 of rat TrpC4
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Antibody s full descriptionMouse Anti-Rat TRPC4 Monoclonal IgG1 Antibody, Clone: S77-15: Biotin
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameCCE1 Antibody, HTRP4 Antibody, STRPC4 Antibody, TRP4 Antibody, TRPC 4 Antibody, transient receptor potential 4 Antibody
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Raised inMouse
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Antibody s targetTRPC4
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Primary research fieldsNeuroscience, Ion Channels, Transient Receptor Potential Channels, Cell Signaling
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~110kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-315 was sufficient for detection of TrpC4 in 10 µg of COS cell lysate transiently transfected with TrpC4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextTransient receptor potential cation channel, subfamily C, member 4, also known as TRPC4, is a human gene encoding a protein of the same name. They are expressed in smooth muscle and endothelial cells where they regulate membrane potential and calcium influx. TrpC4 is activated by G(q)/phospholipase C-coupled receptors, but the underlying mechanism remains elusive (1). Studies suggest TrpC4 contributes to axonal regeneration after nerve injury (2).
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Bibliography1. Otsuguro K., et al. (2008) J Biol Chem. 283(15): 10026-10036. 2. Wu D., Huang W., Richardson P.M., Pristly J.V., Liu M. (2008) J. Biol. Chem. 283(1): 416-426.
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Released date30-Jul-2009
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_001076584.1
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Gene number84494
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Protein numberO35119
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Antibody s datasheetContact our support service
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-TrpC4 Monoclonal Antibody, Clone S77-15 (SMC-315). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpC4 Monoclonal Antibody (SMC-315) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Mouse Anti-TrpC4 Antibody [S77-15] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-315)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolPIRC66, PIRC71, PIRC75, PIRC67, PIRC72, PIRC68, PIRC62, PIRC70, PIRC76, PIRC77
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Short nameMouse Anti- TrpC4 Monoclonal IgG1, Clone S77-15
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelBiotin
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat TrpC4 monoclonal IgG1, clonality S77-15
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Alternative techniquemurine, antibodies
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Clone nameS77-15
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Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 66
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 71
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 75
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 67
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 72
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 68
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 62
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 70
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 76
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 77
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
