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Clone
2C3
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Immunogen
Prepared by immunizing synthetic peptide coupled to KLH.
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Antibody s full description
Mouse Anti-Human Histone H3 Monoclonal IgG1 Antibody, Clone: 2C3: RPE
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
H3 histone family, member A Antibody, H3/A Antibody, H31_HUMAN Antibody, H3FA Antibody, Hist1h3a Antibody, histone 1 H3a Antibody, Histone cluster 1 H3a Antibody, Histone H3.1 Antibody
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Raised in
Mouse
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Antibody s target
Histone H3
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Brandname
None
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Antibodies applications
WB
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody buffer for storage
PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
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Antibody s specificity
Detects endogenous Histone H3 (tri methyl K9) protein.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
A 1:1000 dilution of SMC-254 was sufficient for detection of Histone H3 in 10 µg of Human, Rat, Mouse HeLa, Testis Tissue, Raw 264.7 cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus , Chromosome
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1. Histones play a cental role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
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Bibliography
1. Wu R., et al. (1981) Cell. 27:321. 2. Strahl B., et al. (2000) Nature. 403:41. 3. Bauer U., et al. (2002) EMBO Rep. 3:39. 4. Frank D., et al. (2003) Gene. 312:135.
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Released date
06-Sep-2016
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
P68431/Q71DI3/P84243
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Antibody s datasheet
Contact our support service
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Representative figure legend
Western Blot analysis of Human, Rat, Mouse HeLa, Testis Tissue, Raw 264.7 cell lysates showing detection of ~15 kDa Histone H3 protein using Mouse Anti-Histone H3 Monoclonal Antibody, Clone 2C3 (SMC-254). Lane 1: Human HeLa. Lane 2: Rat Testis Tissue. Lane 3: Mouse Raw 264.7.. Primary Antibody: Mouse Anti-Histone H3 Monoclonal Antibody (SMC-254) at 1:1000. Predicted/Observed Size: ~15 kDa. Mouse Anti-Histone H3 Antibody (tri Methyl Lys9) [2C3] used in Western Blot (WB) on Human, Rat, Mouse HeLa, Testis Tissue, Raw 264.7 cell lysates (SMC-254)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. A methyl group is an alkyl derived from methane, containing one carbon atom bonded to three hydrogen atoms — CH3. In formulas, the group is often abbreviated Me. Such hydrocarbon groups occur in many organic compounds. It is a very stable group in most molecules. While the methyl group is usually part of a larger molecule, it can be found on its own in any of three forms anion, cation or radical. The anion has eight valence electrons, the radical seven and the cation six. All three forms are highly reactive and rarely observed,
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus