Mouse Anti-Human Histone H3 (tri Methyl Lys9) Monoclonal IgG1, Clone 2C3

  • Catalog number
    SMC-254D-A594
  • Price
    Please ask
  • Size
    100 µl
  • Clone
    2C3
  • Immunogen
    Prepared by immunizing synthetic peptide coupled to KLH.
  • Antibody s full description
    Mouse Anti-Human Histone H3 Monoclonal IgG1 Antibody, Clone: 2C3: ATTO 594
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    H3 histone family, member A Antibody, H3/A Antibody, H31_HUMAN Antibody, H3FA Antibody, Hist1h3a Antibody, histone 1 H3a Antibody, Histone cluster 1 H3a Antibody, Histone H3.1 Antibody
  • Raised in
    Mouse
  • Antibody s target
    Histone H3
  • Brandname
    None
  • Antibodies applications
    WB
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000); optimal dilutions for assays should be determined by the user.
  • Antibody buffer for storage
    PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
  • Antibody s specificity
    Detects endogenous Histone H3 (tri methyl K9) protein.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:1000 dilution of SMC-254 was sufficient for detection of Histone H3 in 10 µg of Human, Rat, Mouse HeLa, Testis Tissue, Raw 264.7 cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Nucleus , Chromosome
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1. Histones play a cental role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
  • Bibliography
    1. Wu R., et al. (1981) Cell. 27:321. 2. Strahl B., et al. (2000) Nature. 403:41. 3. Bauer U., et al. (2002) EMBO Rep. 3:39. 4. Frank D., et al. (2003) Gene. 312:135.
  • Released date
    06-Sep-2016
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    Refer to NCBI
  • Gene number
    Refer to GenBank
  • Protein number
    P68431/Q71DI3/P84243
  • Antibody s datasheet
    Contact our support service
  • Representative figure legend
    Western Blot analysis of Human, Rat, Mouse HeLa, Testis Tissue, Raw 264.7 cell lysates showing detection of ~15 kDa Histone H3 protein using Mouse Anti-Histone H3 Monoclonal Antibody, Clone 2C3 (SMC-254). Lane 1: Human HeLa. Lane 2: Rat Testis Tissue. Lane 3: Mouse Raw 264.7.. Primary Antibody: Mouse Anti-Histone H3 Monoclonal Antibody (SMC-254) at 1:1000. Predicted/Observed Size: ~15 kDa. Mouse Anti-Histone H3 Antibody (tri Methyl Lys9) [2C3] used in Western Blot (WB) on Human, Rat, Mouse HeLa, Testis Tissue, Raw 264.7 cell lysates (SMC-254)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. A methyl group is an alkyl derived from methane, containing one carbon atom bonded to three hydrogen atoms — CH3. In formulas, the group is often abbreviated Me. Such hydrocarbon groups occur in many organic compounds. It is a very stable group in most molecules. While the methyl group is usually part of a larger molecule, it can be found on its own in any of three forms anion, cation or radical. The anion has eight valence electrons, the radical seven and the cation six. All three forms are highly reactive and rarely observed,
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Latin name
    Mus musculus
  • Gene target
    Histone   tri   Methyl   Lys9   Monoclonal   IgG1   2C3  
  • Short name
    Mouse Anti- Histone H3 (tri Methyl Lys9) Monoclonal IgG1, Clone 2C3
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    ATTO 594
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse Antibody toHuman Histone H3 (tri Methyl Lys9) monoclonal IgG1, clonality 2C3
  • Alternative technique
    murine, antibodies
  • Clone name
    2C3
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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