Mouse Anti-Human ERp57 Monoclonal IgG2b, Clone 4F9
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Catalog numberSMC-266D-P594
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Price:
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Size100 µg
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Clone4F9
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ImmunogenHuman recombinant ERp57 (Grp58).
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Antibody s full descriptionMouse Anti-Human ERp57 Monoclonal IgG2b Antibody, Clone: 4F9: PE/ATTO 594
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameERp60 Antibody, ERp61 Antibody, Grp57 Antibody, Grp58 Antibody, P58 Antibody, PDIA3 Antibody, PI PLC Antibody, 58 kDa glucose regulated protein Antibody, 58 kDa glucose-regulated protein Antibody, 58 kDa microsomal protein Antibody, Disulfide isomerase ER 60 Antibody, Disulfide isomerase ER-60 Antibody, Endoplasmic reticulum resident protein 57 Antibody, Endoplasmic reticulum resident protein 60 Antibody, ER p57 Antibody, ER protein 57 Antibody, ER protein 60 Antibody, ERp 57 Antibody, ERp57 Antibody, Glucose Regulated Protein 58 Kd Antibody, GRP 57 Antibody, GRP 58 Antibody, GRP57 Antibody, HsT17083 Antibody, p58 Antibody, PDIA 3 Antibody, PDIA3 Antibody, PDIA3_HUMAN Antibody, Phospholipase C alpha Antibody, PI PLC Antibody, Protein disulfide isomerase A3 Antibody, Protein disulfide isomerase family A member 3 Antibody, Protein disulfide-isomerase A3 Antibody
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Raised inMouse
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Antibody s targetERp57
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Primary research fieldsCell Signaling, Protein Trafficking
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BrandnameNone
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Antibodies applicationsWB, ICC/IF
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Antibody s reactivityHuman
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Antibody s dilutionsWB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~57 kDa protein.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysisA 1:1000 dilution of SMC-266 was sufficient for detection of ERp57 in 15 µg of HeLa by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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Antibody in cellEndoplasmic Reticulum , Endoplasmic Reticulum Lumen , Melanosome
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
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Bibliography1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408. 2. Williams D.B. (2005) J Cell Sci. 119: 615-623 3. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889. 4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582. 5. Oliver J.D., et al. (1997) Science 275: 86-88. 6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226. 7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230. 8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305. 9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.
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Released date23-Mar-2017
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_005304.3
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Gene number2923
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Protein numberP30101
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-ERp57 Monoclonal Antibody, Clone 4F9 (SMC-266). Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ERp57 Monoclonal Antibody (SMC-266) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic reticulum, Melanosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ERp57 Antibody (D) Composite. Western Blot analysis of Human Cervical Cancer cell line (HeLa) showing detection of 57 kDa Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone 4F9 (SMC-266). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (SMC-266) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 57 kDa. Mouse Anti-ERp57 Antibody [4F9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (Human Cervical Cancer) (SMC-266) Mouse Anti-ERp57 Antibody [4F9] used in Western Blot (WB) on Human Cervical Cancer cell line (HeLa) (SMC-266)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Short nameMouse Anti- ERp57 Monoclonal IgG2b, Clone 4F9
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2b, IgG2b
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LabelPE/ATTO 594
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman ERp57 monoclonal IgG2b, clonality 4F9
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Alternative techniquemurine, antibodies
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Clone name4F9
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data