Mouse Anti-Human CD74 Monoclonal IgG, Clone PIN.1

  • Catalog number
  • Price
    543.16 USD
  • Size
    100 µg
  • Clone
  • Immunogen
    Human CD74 invariant chain synthetic peptide
  • Antibody s full description
    Mouse Anti-Human CD74 Monoclonal IgG Antibody, Clone: PIN.1: ATTO 633
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    DHLAG Antibody, HLA DR gamma Antibody, HLADG Antibody, p33 Antibody, p35 Antibody, Protein 41 Antibody, CD 74 antibody, CD74 antibody, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody, CD74 antigen antibody, CD74 molecule antibody, CD74 molecule, major histocompatibility complex, class II invariant chain antibody, CLIP antibody, DHLAG antibody, Gamma chain of class II antigens antibody, HG2A_HUMAN antibody, HLA class II histocompatibility antigen gamma chain antibody, HLA DR antigens associated invariant chain antibody, HLA DR gamma antibody, HLA-DR antigens-associated invariant chain antibody, HLA-DR-gamma antibody, HLADG antibody, HLADR antigens associated invariant chain antibody Ia antigen associated invariant chain antibody, Ia antigen-associated invariant chain antibody, Ia associated invariant chain antibody, Ia gamma antibody, Ii antibody, Invariant polypeptide of major histocompatibility complex class II antigen associated antibody, la-gamma antibody, Major histocompatibility complex class II invariant chain antibody, MHC HLA DR gamma chain antibody, MHC HLA-DR gamma chain antibody, p33 antibody, p35 antibody, Protein 41 antibody
  • Raised in
  • Antibody s target
  • Primary research fields
    Immunology, Adaptive Immunity, B Cells, B Cell CD Markers
  • Brandname
  • Antibodies applications
  • Antibody s reactivity
    Human, Mouse
  • Antibody s dilutions
    WB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.2, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~33-35kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.
  • Storage recommendations
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-116 was sufficient for detection of CD74 in 20 µg of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG: AP as the secondary antibody.
  • Antibody in cell
    Cell Membrane , Endoplasmic Reticulum , Endoplasmic Reticulum Membrane , Golgi Apparatus , Endosome , Lysosome
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).
  • Bibliography
    1. Becker-Hermann, S., Arie, G., Medvedovsky H, Kerem A, and Shachar I. (2005) Mol Bio Cell. 16(11):5061-9. 2. Barrera CA., et al (2005) J Histochem Cytochem 53 (12): 1481-9. 3. Starlets D., et al. (2006) Blood. 107 (12): 4807-4816. 4. Burton JD., et al. (2004). Clin Cancer Res. 10(19): 6606-11. 5. Denzin L.K., Hammond, C. and Cresswell, P. (1996) J. Exp. Med. 184: 2153-2165. 6. Denzin L.K., Robbins N.F., Carboy-Newcomb C. and Cresswell P. (1994) Immunity 1: 595-606.
  • Released date
  • PubMed number
    25035924, 19865478
  • Tested applications
    Western Blot, Western Blot
  • Tested reactivity
    Human, Human
  • NCBI number
  • Gene number
  • Protein number
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 (SMC-116). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (SMC-116) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus. Endosome. Lysosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-CD74 Antibody. (C) Composite. Immunohistochemistry analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 (SMC-116). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (SMC-116) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Beautiful basal to suprabasal staining in epidermis, dermis, hair follicles and muscle. Western Blot analysis of Human N87 cell lysates showing detection of CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 (SMC-116). Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (SMC-116) at 1:1000. Lysates treated with macrophage inhibitory factor (MIF). Courtesy of: Victor E. Reyes, University of Texas Medical Branch, USA. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 (SMC-116). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody (SMC-116) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining. Mouse Anti-CD74 Antibody [PIN 1.1] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-116) Mouse Anti-CD74 Antibody [PIN 1.1] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-116) Mouse Anti-CD74 Antibody [PIN 1.1] used in Western Blot (WB) on Human N87 cell lysates (SMC-116) Mouse Anti-CD74 Antibody [PIN 1.1] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-116)
  • Warning information
  • Country of production
  • Total weight kg
  • Net weight g
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Latin name
    Mus musculus
  • Gene target
    CD74   Monoclonal   PIN  
  • Gene symbol
    CD74, DYNLL1
  • Short name
    Mouse Anti- CD74 Monoclonal IgG, Clone PIN 1
  • Technique
    anti-Human, anti-, Mouse, anti, IgG, antibody to, antibodies, antibodies against human proteins, IgGs, Monoclonals or monoclonal antibodies, mouses
  • Host
  • Isotype
    IgG, IgG
  • Label
    ATTO 633
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse Antibody toHuman CD74 molecule, major histocompatibility complex, class II invariant chain monoclonal Immunoglobulin G, clonality PIN.1
  • Alternative technique
    murine, antibodies, immunoglobulins
  • Alternative to gene target
    CD74 molecule, major histocompatibility complex, class II invariant chain, DHLAG and HLADG and Ia-GAMMA and II, CD74 and IDBG-53594 and ENSG00000019582 and 972, MHC class II protein binding, Cell surfaces, Cd74 and IDBG-150302 and ENSMUSG00000024610 and 16149, BT.46809 and IDBG-646288 and ENSBTAG00000015228 and 613384
  • Clone name
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    dynein light chain LC8-type 1
  • Synonyms gene
  • Synonyms gene name
    • dynein, cytoplasmic, light polypeptide 1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Dyneins, axonemal inner arm I1/f complex subunits
    • Dynein 2 complex subunits
    • Dynein 1 complex subunits
    • Dyneins, axonemal outer arm complex subunits
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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