Goat Anti-Human Nrf2 Polyclonal: PerCP
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Catalog numberSPC-218A-PCP
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Price:
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Size50 µg
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ImmunogenPeptide derived from human Nrf2 protein (a.a. 445-458)
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Antibody s targetHuman Nrf2
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Antibody s full descriptionGoat Anti-Human Nrf2 Polyclonal
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Primary research fieldsCancer, Oxidative Stress, Cell Signaling, Epigenetics, Cardiovascular System, Heart
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameErythroid derived 2 antibody, HEBP1 antibody, like 2 antibody, NF E2 related factor 2 antibody, NF-E2-related factor 2 antibody, NF2L2_HUMAN antibody, NFE2 related factor 2 antibody, NFE2-related factor 2 antibody, NFE2L2 antibody, Nrf 2 antibody, NRF2 antibody, Nuclear factor (erythroid derived 2) like 2 antibody, Nuclear factor antibody, nuclear factor erythroid 2 like 2 antibody, Nuclear factor erythroid 2 related factor 2 antibody, Nuclear factor erythroid 2-related factor 2 antibody, Nuclear factor erythroid derived 2 like 2 antibody
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Verified applicationsWB, ELISA
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Raised inGoat
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Antibody s reactivityHuman, Mouse, Rat, Bovine, Dog, Pig
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Antibody s recommended dilutions for useWB (1:1500), ELISA (1:64000); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromProtein A Purified
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Recommended buffer for storageTBS pH7.3, 0.02% sodium azide, 0.5% BSA
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityAggrephagy associated oxidative stress response transcription factor.
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Storage recommendations4°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:1500 dilution of SPC-218 was sufficient for detection of Nrf2 in 35 µg of human lung lysate by electrochemiluminescence analysis using donkey anti-goat IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasm, Nucleus
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextNrf2 is a transcription factor which plays a role in the oxidative stress response. Under normal cellular conditions, it is sequestered in the cytoplasm by Keap1 and Cullin3. When under oxidative stress, Nrf2 is release from Keap1 by p62 and is translocated to the nucleus where it acts as a transcription factor for the expression of oxidative stress-related products.
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Bibliography1. Itoh K., Wakabayashi N., Katoh Y., Ishii T., Igarashi K., Engel J., et al. (1999) Genes Dev. 13(1): 76-86.
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Released date12/Jun/2014
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NCBI numberNP_006155.17
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Gene number4795
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Protein numberQ16251
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure linkNo Data Available
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.05
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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Latin nameCapra aegagrus hircus
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugatePeridinin chlorophyll (PerCP)
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolNFE2L2, GABPA
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Short nameGoat Anti- Nrf2 Polyclonal: PerCP
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Techniqueanti-Human, Polyclonal, anti-, Percp, anti, Goat, antibody to, antibodies, antibodies against human proteins, goats, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Goat Anti- Nrf2 Polyclonal: PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from StressMark antibodies. Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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IsotypeImmunoglobulin
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LabelPerCP
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SpeciesHuman, Goats, Humans
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Alternative namecaprine Antibody toHuman Nrf2 polyclonal: PerCP
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Alternative techniquepolyclonals, antibodies-percp, antibodies, caprine
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Gene info
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Identity
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Gene
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Long gene nameNFE2 like bZIP transcription factor 2
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Synonyms gene name
- nuclear factor (erythroid-derived 2)-like 2
- nuclear factor, erythroid 2-like 2
- nuclear factor, erythroid 2 like 2
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-03-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Basic leucine zipper proteins
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameGA binding protein transcription factor subunit alpha
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Synonyms gene name
- GA-binding protein transcription factor, alpha subunit (60kD)
- GA binding protein transcription factor, alpha subunit 60kDa
- GA binding protein transcription factor alpha subunit
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-01-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ETS transcription factor family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data