NpFR1, Reversible fluorescence intensity-based redox sensor
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Catalog number
SIH-181-1MG
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Price
Please ask
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Size
1 mg
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Stock availabilitIn Stock
In Stock
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CAS number
No Available. See included datasheet
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Description
Reversible fluorescence intensity-based redox sensor
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Molecular weight
431,45 g/mol
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Primary research fields
Cancer, Oxidative Stress
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Other name
none
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Product category
Small Molecules
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Product type
Redox Probe
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Chemical formula
C23H21N5O4
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Origin
Synthetic
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Purity pourcentage
Contact our support service
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Soluble in
Soluble in DMSO
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Physical appearance
Red Solid
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Safety information
Classification: Caution: Substance not yet fully tested. Safety Phrases: S22 - Do not breathe dust S24/25 - Avoid contact with skin and eyes S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection
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PubChem number
Refer to PubChem
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Scientific context
NpFR1, or napthalimide-flavin redox sensor 1, is a novel flavin molecule that exhibits a greater than 100-fold increase in fluorescence upon oxidation, and is easily reversible. In the oxidative form, is shows a maximum absorption at 395 and 463nm, max admission at 545nm. Treatment with a mild reducing agent (including sodium thiosulfate, sodium cyanoborohydride, DTT and glutathione) gave the reduced form which exhibited 110-fold lower absorbance, and 125-fold lower emission. Re-oxidation to its original fluorescence can be achieved by air or by hydrogen peroxide.
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Bibliography
1. Yeow J., Kaur A.K., Anscomb M.D., and New E.J. (2014) Chem. Commun. 50: 8181-8184.
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Release date
15-Jun-2015
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Representative figure link
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Representative figure legend
Fluorescence behavior of NpFR1 (SIH-181) in the oxidized (black) and reduced (red) forms, using 50 µM. Excitation: 405 or 488 nm (not shown). Emission: 490 – 600 nm, with peak at 545 nm. | Chemical structure of NpFR1 (SIH-181), a reversible fluorescence intensity-based redox sensor. This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (more details | Fluorescence emission of NpFR1 (SIH-181, 5 mM, lex = 405 nm) with the incremental addition of sodium dithionite. All spectra were acquired in HEPES buffer (100 mM, pH 7.4). This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (link | Fluorescence response of NpFR1 (SIH-181) to cycles of oxidation and reduction. Reduction was achieved with sodium dithionite (100mM) followed by re-oxidation with 250mM H2O2. Spectra were recorded 5 min after the addition of reducing and oxidising agents. All spectra were acquired in HEPES buffer (100 mM, pH 7.4). This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (see more | Imaging of NpFR1 (SIH-181) in 3T3-L1 preadipocytes treated with (a) vehicle control (b) H2O2 (100 mM, 2 min), (c) NpFR1 (50 mM, 2 h) and (d) NpFR1 (50 mM, 2 h) followed by H2O2 (100 mM, 2 min). Scale bar represents 50 mm, lex = 405 nm. (e) Integrated emission from 510 nm to 610 nm. Values are the mean ratio generated from the intensity from five fields of cells. Error bars represent standard error measurement (s.e.m.). The layout of this image was modified for optimal display from the original Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (info Fluorescence behavior of NpFR1 (SIH-181), a reversible fluorescence intensity-based redox sensor, in the oxidized and reduced forms. | Chemical structure of NpFR1 (SIH-181), a reversible fluorescence intensity-based redox sensor. | Fluorescence emission of NpFR1 (SIH-181), a reversible fluorescence intensity-based redox sensor. | Fluorescence response of NpFR1 (SIH-181), a reversible fluorescence intensity-based redox sensor, to cycles of oxidation and reduction. | Imaging of NpFR1 (SIH-181), a reversible fluorescence intensity-based redox sensor, in 3T3-L1 preadipocytes.
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
0.25
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Net weight g
0.001
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Test
Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
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Gene target
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Short name
NpFR1, Reversible intensity-based redox sensor
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Alternative name
NpFR1, Reversible fluorescence intensity-based redox sensor
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