TNF (G-308A) SNP-Screen RT PCR test, ready to use 0,2 ml tube format

Group

PCR, polymerase chain reaction
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
Gene

Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.

Gene target

TNF G-308A SNP-Screen test format
Gene symbol

TNF, IGHV3-71, TNFRSF1A, HLA-G, NCAPG, FOLR3, TNFRSF1B, SNRPG, GNAO1, SFTA2
Short name

TNF (G-308A) SNP-Screen RT PCR test, 2 tube format
Technique

RT PCR, tube, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. RT PCR is reverse transcription polymerase chain reaction to quantitate mRNA messenger RNA that after cDNA transcription. Sacace uses this as a fast way to measure gene expression. Sacace tubes
Alternative name

tumor necrosis factor (G-308A) SNP-Screen RT PCR test kit test, ready to use 0,2 milliliter tube supplied
Alternative technique

tests, tubes, dna-amplification
Alternative to gene target

tumor necrosis factor, DIF and TNF-alpha and TNFA and TNFSF2, TNF and IDBG-300259 and ENSG00000232810 and 7124, transcription regulatory region DNA binding, Extracellular, Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926, TNF and IDBG-634161 and ENSBTAG00000025471 and 280943

Identity

HGNC:5621
Gene

IGHV3-71
Long gene name

immunoglobulin heavy variable 3-71 (pseudogene)
Synonyms gene
- IGHV3-G
Synonyms gene name
- immunoglobulin heavy variable 3-71
- immunoglobulin heavy variable 3-G pseudogene (provisional)
- immunoglobulin heavy variable 3-G (provisional)
- immunoglobulin heavy variable 3-71 pseudogene
Synonyms
- IGHV3-g
GenBank acession
- AB019437
Locus

14q32.33
Discovery year

2000-04-04
Entrez gene record

28411
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000151881

Identity

HGNC:11916
Gene

TNFRSF1A
Long gene name

TNF receptor superfamily member 1A
Synonyms gene
- TNFR1
Synonyms gene name
- tumor necrosis factor receptor superfamily, member 1A
Synonyms
- TNF-R
- TNFAR
- TNFR60
- TNF-R-I
- CD120a
- TNF-R55
GenBank acession
- M75866
Locus

12p13.31
Discovery year

1991-01-15
Entrez gene record

7132
Pubmed identfication
- 1655358
- 2158863
RefSeq identity
- NM_001065
Classification
- Tumor necrosis factor receptor superfamily
- CD molecules
VEGA ID

OTTHUMG00000168267
Locus Specific Databases
- INFEVERS: The repertory of Familial Mediterranean Fever (FMF) and Hereditary Inflammatory Disorders Mutations
- LRG_193

Identity

HGNC:24304
Gene

NCAPG
Long gene name

non-SMC condensin I complex subunit G
Synonyms gene name
- non-SMC condensin I complex, subunit G
Synonyms
- FLJ12450
- hCAP-G
- CAP-G
- YCG1
Synonyms name
- chromosome condensation protein G
GenBank acession
- AF331796
Locus

4p15.31
Discovery year

2006-09-04
Entrez gene record

64151
Pubmed identfication
- 10910072
- 11136719
RefSeq identity
- NM_022346
Classification
- Armadillo like helical domain containing
- Condensin I subunits
VEGA ID

OTTHUMG00000128539

Identity

HGNC:11917
Gene

TNFRSF1B
Long gene name

TNF receptor superfamily member 1B
Synonyms gene
- TNFR2
Synonyms gene name
- tumor necrosis factor receptor superfamily, member 1B
Synonyms
- TNFBR
- TNFR80
- TNF-R75
- TNF-R-II
- p75
- CD120b
GenBank acession
- M32315
Locus

1p36.22
Discovery year

1991-01-15
Entrez gene record

7133
Pubmed identfication
- 2158863
- 8702885
RefSeq identity
- NM_001066
Classification
- CD molecules
- MicroRNA protein coding host genes
- Tumor necrosis factor receptor superfamily
VEGA ID

OTTHUMG00000001829

Identity

HGNC:4389
Gene

GNAO1
Long gene name

G protein subunit alpha o1
Synonyms gene name
- guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
Synonyms
- G-ALPHA-o
Locus

16q13
Discovery year

1988-04-24
Entrez gene record

2775
Pubmed identfication
- 1899283
- 11395521
RefSeq identity
- NM_020988
Classification
- G protein subunits alpha, group i
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000133241

Name

Polymerase Chain Reaction
Concept

Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Tree numbers
- E05.393.620.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data