sFas Ligand Recombinant Protein

• Catalog number
  40-531
• Price
  Please ask
• Size
  0.002 mg, 0.01 mg

• About recombinant protein
  Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells. Recombinant human soluble Fas Ligand is a 17.9 kDa protein comprising the TNF homologous region of FasL and contains an 8 residue N-terminal His-Tag.
• Other name of the recombinant
  APTL, FASL, CD178, CD95L, ALPS1B, CD95-L, TNFSF6, APT1LG1, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, APTL
• Recombinant in
  CHO cells
• SEQUENCE
  HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL
• Activity
  Biological activity was determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ≤ 10.0 ng/mL, corresponding to a specific activity of ≥ 1 x 105 units/mg.
• Keep at
  The lyophilized sFas Ligand recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted sFas Ligand is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing sFas Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  4557329
• Accession nr
  NP_000630.1
• Pureity
  Greater than or equal to 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  356
• Gene name
  Fas ligand (TNF superfamily, member 6)
• Source
  Homo sapiens
• Uniprot
  P48023
• Pictures of recombinant
• Rec pdf
• Description
  FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  sFas   Ligand   Protein  
• Gene symbol
  FASLG
• Short name
  sFas Ligand Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  sFas Ligand Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:11936
• Gene
  FASLG
• Long gene name
  Fas ligand
• Synonyms gene
  • APT1LG1
  • TNFSF6
• Synonyms gene name
  • tumor necrosis factor (ligand) superfamily, member 6
  • Fas ligand (TNF superfamily, member 6)
• Synonyms
  • FasL
  • CD178
• GenBank acession
  • U11821
• Locus
  1q24.3
• Discovery year
  1994-12-09
• Entrez gene record
  356
• Pubmed identfication
  • 7826947
  • 9022072
• Classification
  • Tumor necrosis factor superfamily
  • Death inducing signaling complex
  • CD molecules
• VEGA ID
  OTTHUMG00000034841
• Locus Specific Databases
  • FASLGbase: Mutation registry for Autoimmune lymphoproliferative syndrome, type 1B (ALPS1B) (previously known as TNFSF6base)
  • LRG_58

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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