sCD100 Recombinant Protein

  • Catalog number
    40-530
  • Price
    Please ask
  • Size
    0.005 mg, 0.02 mg
  • About recombinant protein
    The Semphorins are a large family of phylogenetically conserved proteins that play a pivotal role in maintaining homeostasis in the immune system. Twenty members of this family have been identified and categorized into eight subclasses based on sequence similarity and distinctive structural features. CD100, also known as Sema4D, is a 150 kDa transmembrane class IV semaphorin. Studies have shown that CD100 can induce monocyte migration, T-cell activation, and B-cell survival, as well as T/B cell and T/DC "cooperation”. The CD100 precursor contains 862 amino acids, including a 21 a.a. signal sequence, a 713 a.a. extracellular domain, a 21 a.a transmembrane sequence, and a 107 a.a cytoplasmic region. The extracellular sequence contains several structural features, including a 479 a.a "sema” domain, a 79 a.a. Ig-like sequence, and a 52 a.a "Plexin-type repeat”. Recombinant soluble CD100 is a 78.9 kDa protein comprising the extracellular domain of CD100 (711 amino acids). SDS-PAGE analysis run under non-reducing conditions shows a mixture of disulfide linked dimer and monomer.
  • Other name of the recombinant
    CD100, SEMAJ, coll-4, C9orf164, M-sema-G, CD100, Semaphorin-4D, A8
  • Recombinant in
    CHO cells
  • SEQUENCE
    FAPIPRITWE HREVHLVQFH EPDIYNYSAL LLSEDKDTLY IGAREAVFAV NALNISEKQH EVYWKVSEDK KAKCAEKGKS KQTECLNYIR VLQPLSATSL YVCGTNAFQP ACDHLNLTSF KFLGKNEDGK GRCPFDPAHS YTSVMVDGEL YSGTSYNFLG SEPIISRNSS HSPLRTEYAI PWLNEPSFVF ADVIRKSPDS PDGEDDRVYF FFTEVSVEYE FVFRVLIPRI ARVCKGDQGG LRTLQKKWTS FLKARLICSR PDSGLVFNVL RDVFVLRSPG LKVPVFYALF TPQLNNVGLS AVCAYNLSTA EEVFSHGKYM QSTTVEQSHT KWVRYNGPVP KPRPGACIDS EARAANYTSS LNLPDKTLQF VKDHPLMDDS VTPIDNRPRL IKKDVNYTQI VVDRTQALDG TVYDVMFVST DRGALHKAIS LEHAVHIIEE TQLFQDFEPV QTLLLSSKKG NRFVYAGSNS GVVQAPLAFC GKHGTCEDCV LARDPYCAWS PPTATCVALH QTESPSRGLI QEMSGDASVC PDKSKGSYRQ HFFKHGGTAE LKCSQKSNLA RVFWKFQNGV LKAESPKYGL MGRKNLLIFN LSEGDSGVYQ CLSEERVKNK TVFQVVAKHV LEVKVVPKPV VAPTLSVVQT EGSRIATKVL VASTQGSSPP TPAVQATSSG AITLPPKPAP TGTSCEPKIV INTVPQLHSE KTMYLKSSDN R
  • Activity
    Endotoxin level is less than 0.1 ng per ug (1EU/μg).
  • Keep at
    The lyophilized sCD100 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted sCD100 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing sCD100 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
  • Rec PROTEIN GI
    214010218
  • Accession nr
    NP_006369.3
  • Pureity
    Greater than 97% by SDS-PAGE gel and HPLC analyses.
  • About
    This product is for research use only.
  • Protein s origin
    Human
  • Vial
    Lyophilized
  • NCBI ID
    10507
  • Gene name
    sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
  • Source
    Homo sapiens
  • Uniprot
    Q92854
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    sCD100   Protein  
  • Gene symbol
    SEMA4D
  • Short name
    sCD100 Recombinant Protein
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Alternative name
    sCD100 Rec. Protein
  • Alternative technique
    rec
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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