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Lysates properties
Lymph Node tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
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Lysate s origin
Tissue
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State of tissue
Normal
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Species of the lysate
Human
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Cellular origibn of the lysate
Lymphnode
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Disease of the lysate
Normal
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Localisation of the biopsy
Human lymph node tissue
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Positive control in
the lysate is used as positive control in Western Blot
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PH and medium
Lymph Node lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.
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Keep at
Store at 2-8 grades C for continuous use. For extended storage, freeze working aliquots at -70 grades C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
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Caution
This product is intended for laboratory research purposes only and should be used by qualified personnel only. GENTAUR lysates are not intended for use in humans. GENTAUR is not liable for damages or injuries resulting from receipt and/or use of GENTAUR lysates.
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Laboratory requirements
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. GENTAUR is not liable for damages or injuries resulting from receipt and/or use of GENTAUR materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. Also, for further information on the biosafety classification of human etiologic agents, please consult the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention's Office of Health and Safety (www.cdc.gov/od/ohs).
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Animal origin
Human
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Dilution
2 mg/mL
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