anti-ICAD Antibody
-
Catalog number2001
-
PricePlease ask
-
Size0.02 mg, 0.1 mg
-
-
Gene infoICAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which is cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.
-
Gene namesICAD Antibody: ICAD, DFF35, Dff45, ICAD-L, ICAD-S, A330085O09Rik, Icad, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, DFF-45
-
Antibodie s labelunlabelled serum
-
PurityICAD Antibody is affinity chromatography purified via peptide column.
-
Ab raised inRabbit
-
TypePolyclonal
-
ImmunoglobulinIgG
-
AntigenICAD antibody was made against a peptide corresponding to amino acids near the amino terminus of mouse ICAD.
-
Immuno applicationELISA, Western Blot, immuno-histo-chemistry, immuno fluorescence
-
Antibody adviceICAD antibody can be used for detection of of ICAD by Western blot at 1 ug/mL. A 45 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 ug/mL. For immunofluorescence start at 10 ug/mL.
-
Suspention pH azideICAD Antibody is supplied in PBS containing 0.02% sodium azide.
-
Keep atICAD antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
-
Works withMouse
-
Omim nr9087146
-
Ncbi nrO54786
-
KDa45 kDa
-
Vial with antibodyin solution
-
NCBI GENE nr13347
-
GENE symbolDffa
-
Antigene nameDNA fragmentation factor, alpha subunit
-
SourceMus musculus
-
Protein nrO54786
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translationanticorps
-
Gene target
-
Gene symbolDFFA
-
Short nameanti-ICAD Antibody
-
TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
-
Alternative nameAntibody toICAD (antibody to-)
-
Alternative techniqueantibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nameDNA fragmentation factor subunit alpha
-
Synonyms gene name
- DNA fragmentation factor, 45 kD, alpha polypeptide
- DNA fragmentation factor, 45kDa, alpha polypeptide
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1997-10-27
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data