Anti-CD29 (Stem Cell Marker)(12G10), CF740 conjugate

• Description:

  Recognizes CD29. Binds to the cell membrane of T- and B- lymphocytes, monocytes, platelets, fibroblasts, endothelial cells, mast cells and smooth muscle cells. CD29 is an adhesion receptor and co-stimulatory molecule. This antigen was initially characterized as GPIIa on platelets and as the common b1 subunit of the very late antigen (VLA) protein family. CD29 forms a non-covalent heterodimeric complex with integrin alpha subunits. It also mediates adhesion to invasion and thrombospondin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Fibrinogen Receptor beta subunit; FNRB; Glycoprotein IIa (GPIIA); Integrin beta-1 (ITGB1); integrin VLA-4 beta subunit; MDF2; MSK12; Very Late Activation Protein beta Polypeptide; VLA-4 subunit beta; VLA-BETA; VLAB

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  ITGB1

• Gene ID:

  3688

• NCBI Gene ID:

  643813

• UniProt:

  P05556

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Purified human beta 1 integrin from HT1080 fibrosarcoma cell extract.

• Target Antigen:

  CD29

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  12G10

• Conjugation:

  CF740

• Source:

  Animal

• Applications:

  Flow, surface (published) | Functional studies (published) | IF (published) | IP (published)

• Validated Applications:

  FC, IF, IP

• Positive Control:

  HT1080 whole cell lysate. HeLa cell line.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% rBSA, 0.05% azide

• Molecular Weight:

  110 kDa (non-reduced) & 130 kDa (reduced)

• Additionnal Information:

  Does not react with Mouse or Rat; ELISA: For coating use Ab at 1-5 ug/mL, order Ab without BSA; Optimal dilution for a specific application should be determined by user.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. FEBS Lett (1995) 363: 118-122. (functional studies) J Cell Sci (2005) 118: 4009-4016. (IP) Traffic (2012) 13: 610-625. (Flow, IF)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4