Goat anti-NSP1 (SARS-CoV-2) Polyclonal antibody

• Description:

  Host translation inhibitor Nsp1 is part of the multifunctional protein replicase polyprotein 1ab that is involved in the transcription and replication of viral RNA. NSP1 inhibits the host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Therefore, Nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response by suppressing host gene expression.

• Specifications:

  In lysates of transfected cells with the plasmid containing the sequence used, detects the fusion protein by Western blot.

• Product Name Alternative:

  Host translation inhibitor, Nsp1 SARS Coronavirus-2 antibody.

• Volume:

  100 µL

• Host:

  Goat

• Antigen Species:

  SARS-CoV-2

• Reactivity:

  Reacts with SARS-CoV-2 protein

• Immunogen:

  Affinity purified recombinant fusion protein Nsp1 of SARS-CoV-2 produced in E. coli.

• Target Antigen:

  Affinity purified recombinant fusion protein Nsp1 of SARS-CoV-2 produced in E. coli.

• Immunogen Type:

  Recombinant protein

• Target:

  anti-NSP1 (SARS-CoV-2)

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Type:

  Primary

• Sequence:

  MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGG

• Applications:

  WB

• Purification:

  Epitope affinity purified

• Concentration:

  3 mg/mL

• Dilution:

  WB:1:500-1:2,000

• Form:

  Polyclonal antibody supplied as a 100 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

• Buffer:

  PBS, 20% glycerol and 0.05% sodium azide

• Storage Conditions:

  For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

• CAS Number:

  9007-83-4