Anti-Streptavidin from Streptomyces avidinii

• Description:

  This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

• Reactivity:

  Other

• Immunogen:

  Chromatography on protein A Sepharose

• Clonality:

  Monoclonal

• Type:

  Antibodies-Monoclonal

• Applications:

  WB,IHC,ELISA,ICC/IF,Flow cyt

• Concentration:

  1ug/ul

• Purity:

  Chromatography on protein A Sepharose

• Buffer:

  PBS, pH 7.4 with 0.05% sodium azide.

• Shipping Conditions:

  Ice Pack

• Storage Conditions:

  This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

• Fragment:

  IgG1, kappa

• Specificity:

  No cross-reactivity with egg white avidin. Affinity (Kd) > 1 x 108 for all MAbs. MAbs bind with high efficiency with different epitopes of streptavidin molecule. MAbs bind to the streptavidin without interfering with the biotin-binding reaction.

• Applications Notes:

  MAbs can be used to localize streptavidin, to amplify signals in immunochemistry, immunohistochemistry, ELISA, flow cytometry and immunoblotting. These mouse anti-streptavidin MAbs applied to the ABC complex, previously bound to the second antibody system allow for further enhancement of signal when anti-mouse antibody conjugate is added. MAbs are effective in immunoadsorbtion procedures of streptavidin - biotinylated protein complexes.

• CAS Number:

  9007-83-4