Anti-Smooth Muscle Actin(ACTA2/791)

• Description:

  Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  ACTA2|Actin Alpha 2 Smooth Muscle Aorta|Actin Alpha Smooth Muscle|Actin Aortic Smooth Muscle|Actin Vascular Smooth Muscle|ACTSA|ACTVS|Alpha 2 Actin|Alpha Cardiac Actin|Alpha-actin-2|ASMA|Cell Growth-inhibiting Gene 46 Protein|Growth Inhibiting Gene 46

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  ACTA2

• Gene ID:

  59

• NCBI Gene ID:

  500483

• UniProt:

  P62736

• Cellular Locus:

  Cytoskeleton

• Host:

  Mouse

• Species Reactivity:

  Human, Rat

• Immunogen:

  Recombinant full-length human ACTA2 protein

• Target Antigen:

  Smooth Muscle Actin

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  ACTA2/791

• Conjugation:

  Purified, BSA-free

• Source:

  Animal

• Applications:

  IF (verified) | IHC, FFPE (verified)

• Validated Applications:

  IF, IHC, FFPE

• Field of Research:

  Cytoskeleton

• Positive Control:

  Blood vessels in all tissues, smooth muscle or leiomyosarcoma

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  42 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Predicted to show broad reactivity|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4