S100A9 (47-8D3), CF594 conjugate, 0.1mg/mL

This MAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer's cells of the liver and Langerhan's cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) . It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

9007-83-4

S100-A9; MRP14; calgranulin-B; calprotectin L1H subunit; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14; S100 calcium-binding protein A9 (calgranulin B)

41116161

Primary and secondary antibodies for multiple methodology immunostaining detection application

S100A9

6280

112405

P06702

P06702

S100A9

Human peripheral blood monocyte components

S100A9

47-8D3

Primary

Animal

Animal

WB (published)

Inflammation

Liver or histiocytoma

2 years

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

14 kDa

Note: References for this clone sold by other suppliers may be listed for expected applications. J Invest Dermatol (2010) 130(10) 2423-2430. (WB)

RT (ND)

Stable at room temperature or 37°C (98°F) for 7 days.

Protect from light

Guaranteed for at least 24 months from date of receipt when stored as recommended

IgG1, kappa

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

0.05% sodium azide (CAS 26628-22-8)