Anti-NGFR (Nerve Growth Factor Receptor)(NGFR5)

• Description:

  This antibody recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  CD271; Gp80-LNGFR; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Nerve growth factor receptor (NGFR); p75 ICD; p75 Neurotrophin receptor; Tumor necrosis factor receptor superfamily member 16 (TNFRSF16)

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  NGFR

• Gene ID:

  4804

• NCBI Gene ID:

  415768; 681726

• UniProt:

  P08138

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Baboon, Cat, Ferret, Human, Monkey, Rabbit

• Immunogen:

  NGFR from A875 melanoma cells

• Target Antigen:

  NGFR

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  NGFR5

• Conjugation:

  Purified, with BSA

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Cancer, Developmental biology, Signal transduction

• Positive Control:

  Melanoma|Neuronal axons, Schwann cells, and perineural cells of peripheral nerves|Soma and axons of sensory neurons, and ganglionic satellite cells|Tumors of nerve sheath differentiation, Schwannoma, Neurofibroma

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  75 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Does not react with mouse or rat, others not known|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4