Anti-Ep-CAM / CD326(VU-1D9)

• Description:

  This antibody reacts with the first EGF repeat in the extracellular domain of Ep-CAM. It is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  TACSTD1

• Gene ID:

  4072

• NCBI Gene ID:

  542050

• UniProt:

  P16422

• Cellular Locus:

  Exosomes/EVs|Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Small cell lung carcinoma cells

• Target Antigen:

  CD326 | Ep-CAM

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  VU-1D9

• Conjugation:

  Purified, BSA-free

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Exosome staining (verified) | Flow, surface (verified) | IF (verified) | IHC, FFPE (verified) | WB (published)

• Validated Applications:

  FC, IF, IHC, FFPE, WB

• Field of Research:

  Cancer, Cell adhesion, Exosomes/EVs

• Positive Control:

  HT29 cells or breast tumor

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  40-43 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Does not react with rat or ferret; others not known|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Mol Cell Biol 21(7): 2570-2580. (western; epitope mapping)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4