Rat FFAR2 (Free Fatty Acid Receptor 2) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FFAR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FFAR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FFAR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FFAR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  FFA2R; GPR43; FFA2; GPCR43; G-protein coupled receptor 43

• Gene Aliases:

  FFA2R; GPR43; FFA2; GPCR43; G-protein coupled receptor 43

• UniProt:

  Q76EI6

• Reactivity:

  Rat

• Field of Research:

  Metabolic pathway

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FFAR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FFAR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FFAR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FFAR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  10 ng/mL

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids.

• Detection Range:

  0.16 - 10 ng/mL

• Sensitivity:

  0.067 ng/mL

• Species:

  Rat

• CAS Number:

  7732-18-5