Human KDM4A (Lysine Specific Demethylase 4A) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KDM4A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KDM4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KDM4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KDM4A in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  JMJD2; JMJD2A; JHDM3A; JmjC domain-containing histone demethylation protein 3A; Jumonji domain-containing protein 2A

• Gene Aliases:

  JMJD2; JMJD2A; JHDM3A; JmjC domain-containing histone demethylation protein 3A; Jumonji domain-containing protein 2A

• UniProt:

  O75164

• Reactivity:

  Human

• Field of Research:

  Enzyme & Kinase

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KDM4A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KDM4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KDM4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KDM4A in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  20 ng/mL

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids.

• Detection Range:

  0.32 - 20 ng/mL

• Sensitivity:

  0.128 ng/mL

• Species:

  Human

• CAS Number:

  7732-18-5