Anti-Creatine Phosphokinase(2ba6)

• Description:

  Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments. They provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteinsPrimary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  B-CK; BB-CK; creatine kinase-B; Creatine kinase B chain; CKBB creatine kinase B-type; creatine kinase; brain; CKBB; Brain creatine kinase; Creatine kinase B-type; Creatine Kinase BB Isoenzyme; Creatine kinase brain; Creatine phosphokinase BB

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CKB

• Gene ID:

  1152

• NCBI Gene ID:

  173724

• UniProt:

  P12277

• Cellular Locus:

  Cytoplasmic

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human CKBB protein

• Target Antigen:

  Creatine Phosphokinase

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  2ba6

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Field of Research:

  Metabolism

• Positive Control:

  Cerebellum

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  43 kDa (Monomer); 86 kDa (Dimer)

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Not suitable for FFPE sections|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4