Rat SPB (Surfactant Associated Protein B) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SPB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SPB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SPB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SPB in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  SFTPB; PSPB; SFTB3; SFTP3; SP-B; Pulmonary Surfactant Protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein

• Gene Aliases:

  SFTPB; PSPB; SFTB3; SFTP3; SP-B; Pulmonary Surfactant Protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein

• UniProt:

  P22355

• Reactivity:

  Rat

• Field of Research:

  Tumor immunity, Infection immunity, Pulmonology

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SPB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SPB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SPB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SPB in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  10 ng/mL

• Sample Type:

  Tissue homogenates, cell lysates, lung lavage fluid and other biological fluids

• Detection Range:

  0.16 - 10 ng/mL

• Sensitivity:

  0.054 ng/mL

• Species:

  Rat

• CAS Number:

  7732-18-5